双水相法萃取植物乳杆菌M1-UVs29细菌素条件的优化  

Optimization of Aqueous Two-phase Extraction of Bacteriocin by Lactobacillus plantarum M1-UVs29

作　　者：梁小月 张东杰[1] 王颖[1,2] 王月[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319 [2]国家杂粮工程技术研究中心

出　　处：《黑龙江八一农垦大学学报》2016年第2期53-59,共7页journal of heilongjiang bayi agricultural university

基　　金：防腐保鲜新型物流包装材料开发(2015BAD16B05)

摘　　要：为优化植物乳杆菌M1-UVs29细菌素的萃取条件,选择合适的成相物质后,通过单因素试验考察不同PEG浓度、硫酸铵浓度、p H、离子强度等对细菌素效价及萃取率的影响。在此基础上,利用响应面进行优化。结果表明:最佳萃取条件是20%PEG4000,18%硫酸铵,1.3%氯化钠,体系p H4.0,效价1 258.01±14.23 IU·m L-1,萃取率达到83.7%。说明双水相法可以较好地纯化植物乳杆菌M1-UVs29细菌素。To optimize of extraction conditions of Lactobacillus plantarum M1-UVs29 bacteriocin,the suitable phase material was chosen to test effects of different concentration of PEG,ammonium sulfate,p H,ionic strength on bacteriocin titer and extraction rate by the single factor experiment. The results showed that the optimum extraction conditions were 20% PEG4000,18% ammonium sulfate,1.3% sodium chloride,p H4.0,titer 1 258.01 ±14.23 IU·m L-1,and the rate of extraction achieved by 83.7%. It proved that aqueous two-phase systems could well purify bacteriocins by Lactobacillus Plantarum M1-UVs29.

关 键 词：植物乳杆菌 细菌素 双水相 响应面 

分 类 号：Q93[生物学—微生物学]