复原乳中三种常见致病菌的生长动力学模型构建  

Growth kinetic model developed for three common pathogenic bacteria in rehydrated infant milk formula

作　　者：邱红玲 唐晓阳 王李伟[2] 储瑞蔼 彭少杰[2] 

机构地区：[1]上海生命科学研究院营养科学研究所,上海200233 [2]上海市食品药品监督管理局执法总队,上海200233

出　　处：《上海预防医学》2016年第5期288-293,共6页Shanghai Journal of Preventive Medicine

基　　金：2011年上海市科委重点科技攻关项目(11391902000)

摘　　要：目的建立单核细胞增生李斯特菌、阪崎肠杆菌和蜡样芽胞杆菌在复原乳中的生长动力学模型。方法将3种致病菌分别接种于复原乳中,分别在低温（4℃、8℃）、适温（25℃、37℃和42℃）条件下储藏不同时间,参考国标GB 4789.2—2010对菌落总数进行计数,采用修正的Gompertz模型和平方根模型模拟3种致病菌在低温和适温条件下的一级和二级生长动力学模型,并验证模型的有效性。结果修正的Gompertz模型能较好地拟合3种致病菌的生长曲线,拟合的R2范围在0.97~1.00。平方根模型能较好地拟合温度与3种致病菌最大比生长速率μmax的线性关系,其R2范围为0.93~0.99之间,经验证3种细菌的生长动力学模型的Bf和Af均在可接受范围内。结论本研究建立了复原乳中3种致病菌一级和二级生长动力学模型,该模型为开展复原乳中3种致病菌的风险评估和预警提供了理论基础。Objective To develop the kinetic model and study the growth parameters of listeria monocytogenes,enterobacter sakazakii and bacillus cereus in the rehydrated infant milk formula stored at different temperatures and time periods. Methods The three pathogenic bacteria were inoculated respectively in the rehydrated infant milk formula,and stored at 4℃,8℃,25℃,37℃ and 42℃ separately for different periods of time. The numbers of bacteria colony were counted referring to national standards（ GB 4789. 2- 2010）. A modified Gompertz model and root square model were used to estimate the primary and secondary growth parameters of three pathogenic bacteria in the rehydrated infant milk formula. The validity of the model was further verified. Results Modified Gompertz model fitted well with the growth curves of three pathogenic bacteria in the rehydrated infant milk formula respectively,with the range of R2 between 0. 97 and 1. 00. Good linear fitting was found between temperature and μmax（ R2：0. 93- 0. 99） of the three pathogenic bacteria by using root square model. Further validation of the developed models indicated that the value of Bfand Afwere acceptable. Conclusion The primary and second growth kinetic models were developed for three bacteria respectively in the rehydrated infant milk formula. This study contributes to providing theoretical basis for the risk assessment and early warning of the three pathogenic bacteria in rehydrated infant milk formula.

关 键 词：复原乳 李斯特菌 阪崎肠杆菌 蜡样芽胞杆菌 Gompertz模型 平方根模型 

分 类 号：R155.5[医药卫生—营养与食品卫生学]