柠檬烯对食源性病原菌的抑菌活性（英文）  

Antibacterial effect of limonene on food-borne pathogens

作　　者：卢海燕[1] 徐重新[1] 张宵[1] 梁颖[1] 刘贤金[1] 

机构地区：[1]江苏省农业科学院江苏省食品质量安全重点实验室农业部农产品质量安全控制技术与标准重点实验室,南京210014

出　　处：《浙江大学学报（农业与生命科学版）》2016年第3期306-312,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：supported by the National Natural Science Foundation of China(31201356);The Agricultural Science and Technology Innovation in Jiangsu Province(cx(14)2060);the Special Fund for Agro-scientific Research in the Public Interest of China(201303088)

摘　　要：研究了柠檬烯乳化液以及柠檬烯丙酮溶液对3种食源性病原菌的抑菌活性。滤纸片扩散试验表明,对小肠结肠炎耶尔森菌Yersinia enterocolitica的抑菌活性高低为氧氟沙星(阳性对照)>500μL/L柠檬烯丙酮溶液>5 000μL/L柠檬烯丙酮溶液>柠檬烯乳化液>50μL/L柠檬烯丙酮溶液>丙酮(溶剂对照),抑菌圈分别为46.8mm,32.2mm,27.4mm,17.1mm,14.5mm和9.2mm;对金黄色葡萄球菌Staphylococcus aureus活性最高的是阳性对照,其次是50μL/L柠檬烯丙酮溶液,抑菌圈达30.3 mm;柠檬烯丙酮溶液对单核增生李斯特菌Listeria monocytogenes具有抑菌圈,但与丙酮的抑菌圈大小相似,而柠檬烯乳化液未表现出抑菌活性,说明柠檬烯对该菌没有抑制作用,溶剂丙酮具有一定的抑菌活性。试管梯度试验表明,柠檬烯乳化液和柠檬烯丙酮溶液对小肠结肠炎耶尔森菌和金黄色葡萄球菌具有抑菌活性,对单核增生李斯特菌没有活性,且柠檬烯丙酮溶液的最小抑菌浓度和最小杀菌浓度远低于柠檬烯乳化液,说明前者的抑菌活性更好。所选3种食源性病原菌中,小肠结肠炎耶尔森菌对柠檬烯最为敏感,金黄色葡萄球菌次之,单核增生李斯特菌最不敏感。Summary The effects of limonene emulsion and limonene acetone solution on three species of food-borne pathogens were studied. The results of the agar diffusion test indicated that the order of the antibacterial effect on Yersinia enterocolitica was ofloxacin （positive control） 〉 500 μL/L limonene acetone solution 〉 5000 μL/L limonene acetone solution 〉 limonene emulsion 〉 50 μL/L limonene acetone solution 〉 acetone （solvent control）, with the inhibition zone diameters of 46.8, 32.2, 27.4, 17.1, 14.5, 9.2 mm, respectively. Except the positive control, the most effective one on Staphylococcus aureus was 50 μL/L limonene acetone solution （the inhibition zone diameter was 30.3 mm）. The inhibition zones of limonene acetone solution and acetone to Listeria monocytogenes were similar, while there was no inhibition zone of limonene emulsion, which indicated that it was acetone but not limonene that had the antibacterial activity to L. monocytogenes. The tube dilution test showed that limonene was effective to Y. enterocolitica and S. aureus, but un- effective to L. monocytogenes. Limonene acetone solution showed a higher antibacterial activity with lower values of minimal inhibitory concentration and minimal bactericide concentration than limonene emulsion. Among the three selected food-borne pathogens, Y. enterocolitica was the most sensitive one to limonene, followed by S. aureus and L. mtmoc ytogenes .

关 键 词：柠檬烯 乳化 抑菌活性 食源性病原菌 

分 类 号：R378[医药卫生—病原生物学] TS201.3[医药卫生—基础医学]