糙米中黄曲霉毒素B_1的ATR-FTIR快速测定  

Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy(ATR-FTIR) for Rapid Detection of Aflatoxin B_1 in Brown Rice

作　　者：沈飞[1] 吴启芳[1] 唐培安[1] 邵小龙[1] 姜大峰[2] 

机构地区：[1]南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,江苏南京210023 [2]山东省疾病预防控制中心,山东济南250014

出　　处：《食品科学》2016年第12期187-191,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(31301482);江苏省青年自然科学基金项目(BK20131007);江苏省高校自然科学研究项目(13KJB550009);江苏高校优势学科建设工程资助项目

摘　　要：拟建立基于衰减全反射-傅里叶变换红外光谱（attenuated total reflectance-Fourier transform infrared spectroscopy,ATR-FTIR）的糙米中微量黄曲霉毒素B1（aflatoxin B1,AFB1）的快速检测方法。首先将16份糙米样品经辐射灭菌后接种产毒菌株并在适宜条件下（28℃、相对湿度85%）贮藏30 d,测定其AFB1含量。其次将受AFB1污染样品磨粉后与空白糙米样品按质量比例进行精确混合,获得AFB1含量在0-890.20μg/kg内的糙米样品共计128份。ATR-FTIR建模分析结果显示,依据AFB1含量高低将样品划分为4类,临近算法判别分析模型的预测整体正确率达到83.3%。运用偏最小二乘回归分析建立的定量模型精度最优,预测相关系数、均方根误差和相对分析误差值分别为0.970、70.8μg/kg和4.0。结果表明,ATR-FTIR技术用于糙米黄曲霉毒素污染程度的快速分析与筛选具有一定可行性,但需进一步研究来提升对低AFB1含量样品的预测精度。The objective of this work was to establish a rapid method for aflatoxin B1 detection in brown rice by attenuatedtotal reflectance-Fourier transform infrared spectroscopy （ATR-FTIR）. A set of 16 brown rice samples were sterilized withradiation, incubated after inoculation with toxigenic strains and stored under appropriate conditions （28 ℃ and 85% RH）for 30 days, and the concentrations of AFB1 were determined. Then the samples contaminated by AFB1 were blended withblank samples in various weight proportions. A total of 128 samples with concentrations ranging from 0 to 890.20 μg/kgwere obtained. The ATR-FTIR calibration results indicated that a classification accuracy of 83.3% was obtained by Knearest neighbors （KNN） when the samples were divided into four groups according to AFB1 concentration. Quantitativeanalysis model was built by partial least square regression （PLSR）, which showed the best performance in predication, andthe regression coefficient of validation （rv）, root mean square error of prediction （RMSEP） as well as RPD value, whichwere 0.970, 70.8 μg/kg and 4.0, respectively. Overall, ATR-FTIR offers a feasible method for rapid screening of brown ricesamples contaminated by AFB1. However, further study is needed to improve the prediction power for samples with lowAFB1 concentrations.

关 键 词：衰减全反射—傅里叶变换红外光谱 糙米 黄曲霉毒素B1(AFB1) 快速检测 

分 类 号：TS272.7[农业科学—茶叶生产加工] O657.3[轻工技术与工程—农产品加工及贮藏工程]