Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的工艺优化  

Optimization on determination of total polyphenol content in sea buckthorn wine by Folin-Ciocalteu colorimetric method

作　　者：牛广财[1] 闫公昕 朱丹[2] 魏文毅[1] 李世燕[1] 刘志明[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《食品与机械》2016年第4期80-83,142,共5页Food and Machinery

基　　金：黑龙江省农垦总局科学技术研究项目(编号:HNK16KF-08)

摘　　要：以没食子酸作为对照品,通过单因素和正交试验,对Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的条件进行优化。结果表明,Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的最优条件为：Folin-Ciocalteu试剂1 mL,蒸馏水5mL,7.5%Na_2CO_3溶液3mL,在30℃下避光反应1h后,测定其在735nm处的吸光度,总多酚含量在0~20μg/mL范围内与吸光度值有良好的线性关系。该测定方法的平均加标回收率为100.64%,精密度,稳定性以及回收率的相对标准偏差（RSD）分别为1.46%,0.22%和2.63%。该方法简便、快速,精密度高,稳定性和重复性好,可用于沙棘酒中总多酚含量的测定。Optimizeed the optimal conditions of Folin-Ciocalteucolorimetry,which was used to determine the total polyphenols content in sea buckthorn wine,and the sea buckthorn wine were investigated by orthogonal experiments combined with single factor experiments by using gallic acid as the standard.The results indicated that the total phenol contents of sea buckthorn wine could be well calculated according to their colorimetric absorption at 735 nm by applying FolinCiocalteu reagent 1.0 mL and 5 mL distilled water,7.5%Na_2CO_3 3mL,at 30℃for colorimetric reaction,and there was a good linearity between content of phenol and absorbance in the range of 0~20μg/mL.With this method,the stability,precision and average recovey rate were 1.46%,0.22% and 2.63% by RSD（relative standard deviation）,respectively.And the average recovery rate was100.64%.This method is convenient and fast to determine the total polyphenols of sea buckthorn wine with the advantages of high precision,stability and good repetition.

关 键 词：沙棘酒 总多酚 FOLIN-CIOCALTEU法 

分 类 号：O657.3[理学—分析化学] TS262.7[理学—化学]