不同窖龄窖泥原核生物多样性的高通量测序研究  

High-throughput Sequencing Using in Research for Community Diversity of Prokaryotes in Pit Mud of Different Ages

作　　者：李克亚[1] 文章[1] 邓斌 李周[1] 胡承[1] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [2]重庆市食品药品检验检测研究院,重庆401121

出　　处：《食品工业》2016年第6期121-125,共5页The Food Industry

摘　　要：窖池环境和其中多样的微生物构成了浓香型大曲酒发酵的特殊生态系统,其中蕴藏着丰富的微生物资源。试验通过对两个酒厂的新老窖池的不同取样位置的窖泥样品进行基于Illumina Miseq平台对细菌V3可变区进行高通量测序并结合理化性质的测定,研究各窖泥样品原核生物的群落结构,以及窖泥理化性质和窖泥中所含放线菌丰度的联系。研究结果初步表明,窖池老熟过程本质上是窖泥理化性质与其中微生物群落多样性交互作用的过程。The pit for fermentation of Chinese liquor provides habitat for variety of microbes, which holds in store of abundant microbial resources. High-throughput sequencing based on Illumina Miseq was used to analyze V3 variable regions of prokaryotes from different pit mud samples and find connection between the abundance of actinobacteria and properties of pit mud of different age. It preliminarily shows that the process of pit maturation is essentially the interaction process between pit mud physicochemical properties and microbe diversities.

关 键 词：窖泥 多样性分析 丰度 高通量测序 原核生物 

分 类 号：TS261.1[轻工技术与工程—发酵工程]