表没食子儿茶素没食子酸酯(EGCG)标准样品的研制  

Development of EGCG Reference Material

作　　者：王尉[1,2] 林楠[1,2] 周晓晶[1,2,3] 乐胜锋[1,2] 杜宁[1,2,3] 张经华[1,3] 

机构地区：[1]北京市理化分析测试中心,北京100089 [2]有机材料检测技术与质量评价北京市重点实验室,北京100094 [3]全国标准样品技术委员会天然产物标准样品专业工作组,北京100094

出　　处：《食品科学》2016年第14期110-115,共6页Food Science

基　　金：国家质检总局公益性行业科研发展专项(201210209)

摘　　要：依据GB/T 15000.3—2008《标准样品工作导则(3)标准样品:定值的一般原则和统计方法》,开展表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)国家标准样品研制工作。以绿茶样品作为研究材料,采用溶剂提取、萃取、高速逆流色谱分离纯化,制备得到EGCG。通过紫外-可见吸收光谱、红外光谱、质谱和核磁共振波谱等方法对EGCG样品进行结构鉴定,并进行均匀性、稳定性检验。采用8家实验室进行联合定值,并对定值结果进行数据分析。结果:EGCG标准样品均匀性和稳定性良好,定值结果为99.29%,置信度95%的扩展不确定度为0.16%。结论:所制备得到的EGCG样品满足GB/T 15000.3—2008的要求,可用于绿茶相关产品中EGCG的质量控制和方法验证。Objective： This study aimed to prepare epigallocatechin gallate（EGCG） reference material according to the Directives for the Work of Reference Materials（3）： Reference Materials—General and Statistical Principles for Certification（GB/T 15000.3—2008）. Methods： EGCG from green tea was separated by extraction and high speed counter current chromatography（HSCCC）. Its chemical structure was identified by ultraviolet-visible（UV） spectroscopy, infrared（IR） spectroscopy, mass spectrometry（MS） and nuclear magnetic resonance（NMR） spectroscopy. The homogeneity and stability were systematically checked. A cooperative validation was conducted in 8 laboratories and the results of certification for EGCG were analyzed. Results： The homogeneity and stability of the reference material of EGCG were excellent, and the certified value was 99.29% with an expanded uncertainty of 0.16% at confidence coefficient of 95%. Conclusions： The reference material of EGCG conforms to the requirement of the Directives for the Work of Reference Materials（3） and can be used for validation of analytical methods for quality control of green tea products.

关 键 词：表没食子儿茶素没食子酸酯 绿茶 标准样品 均匀性 稳定性 定值 

分 类 号：TS272.7[农业科学—茶叶生产加工]