嗜酸乳杆菌肽聚糖的六种提取方法比较研究  

Comparison of six extraction methods of peptidoglycan in Lactobacillus acidophilus

作　　者：杨旭[1] 朱静静[1] 潘道东[1] 吴振[1] 曾小群[1] 孙杨赢[1] 曹锦轩[1] 

机构地区：[1]宁波大学食品科学与工程系,浙江宁波315211

出　　处：《食品工业科技》2016年第15期66-70,75,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(41276121;31471598);宁波市重大攻关项目(2011C11017)

摘　　要：比较嗜酸乳杆菌肽聚糖的六种提取方法,得到提取肽聚糖的最适方法并对此方法下提取的肽聚糖进行初步评价。最适提取方法步骤如下:三氯乙酸脱磷壁酸,乙醚脱脂,十二烷基磺酸钠结合胰蛋白酶去除蛋白质,此方法下肽聚糖的平均得率为16.48%。溶菌酶实验及肽聚糖结构测定结果显示提取的肽聚糖为高纯度肽聚糖且电镜下成片状,并保持完整的菌体形态。氨基酸分析结果表明肽聚糖的主要氨基酸组成为丙氨酸、谷氨酸、赖氨酸、天冬氨酸和亮氨酸,为肽聚糖免疫效应的研究提供了理论基础。Six extraction methods of peptidoglycan of Lactobacillus acidophilus were compared and analyzed to get the optimal method, and its product was primarily evaluated. The steps of the optimal extraction way of peptidoglycan were as follows：the phosphate wall acid was removed with trichloroacetic acid （TCA）, then the fat was skimmed with ether, finally, protein was removed with sodium dodecylsulphate （SDS）and trypsin.Under the method,the average extraction output was 16.48%.The results of structure determination and the experiment of lysozyme solution showed that the extracted peptidoglycan was pure.Under scanning electron microscopy（SEM）, the peptidoglycan was schistose and maintained complete cell morphology of Lactobacillus acidophilus.Amino acid analysis suggested that alanine（Ala） and glutamic acid （ Glu）, lysine （ Lys）, aspartic acid （ Asp ） and leucine （Leu） were the principal amino acid components of peptidoglycan and the result would provide a theoretical basis for the study on the immune effect of peptidoglycan.

关 键 词：肽聚糖 嗜酸乳杆菌 提取方法 结构测定 

分 类 号：TS201.1[轻工技术与工程—食品科学]