脱落酸对采后番茄愈伤次生代谢物质合成的影响  

Effect of abscisic acid on secondary metabolite production during wound-healing in postharvest tomato fruit

作　　者：陶晓亚[1] 李家寅[1] 韩雪源 黄斯[1] 茅林春[1] 

机构地区：[1]浙江大学食品科学与营养系,馥莉食品研究院,浙江省农产品加工重点实验室,浙江杭州310058

出　　处：《食品工业科技》2016年第16期330-334,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31372113)

摘　　要：为研究脱落酸(abscisic acid,ABA)对采后番茄愈伤次生代谢物质合成的影响,以绿熟期樱桃番茄为材料,对果实损伤处理后,分别用ABA及其合成抑制剂氟啶酮(fluridone,FLD)抽真空渗透处理,黑暗条件下贮藏愈伤,每天取样测定相关生理指标。测定了果实失重率,伤口部位的总酚、类黄酮含量及相关酶活性,丙二醛(MDA)含量。结果表明:与对照相比,ABA处理减缓果实失重率升高,可有效促进伤口部位总酚、类黄酮的积累,提高与此相关的苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,降低丙二醛(MDA)含量,减少伤口部位氧化损伤,加速伤口愈合;FLD处理则起相反作用,加剧伤口部位氧化损伤,减缓伤口愈合进程。以上结果说明,ABA对采后番茄愈伤次生代谢物质合成具有促进作用。The effect of abscisic acid（ ABA） on wound- healing,secondary metabolite production and related enzyme activities in postharvest mature green cherry tomato fruit was investigated in this study. Tomato fruit were treated under vacuum with ABA and fluridone（ FLD） solution after wounding and allowed to heal in the dark.Weight loss of fruit,total phenol,flavonoids and MDA content,and enzyme activities（ PAL,SOD,CAT） in wound tissues were determined every day during storage time. The results indicated that weight loss and MDA content were reduced in ABA- treated tomato fruit compared with control.ABA promoted wound- healing process by enhancing total phenol and flavonoids contents as well as PAL,SOD and CAT activities in wound tissues of tomato fruit.However,FLD treatment had the opposite effects. In conclusion,ABA promoted the synthesis of secondary metabolite production during wound- healing in postharvest tomato fruit.

关 键 词：脱落醛 采后 番茄 愈伤 次生代谢物质 酶活性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]