等电位间隔-电位滴定法连续测定谷物中脂肪酸值和氨基酸态氮值的实验  

Continuous Determination of Fatty Acid and Amino Acid Nitrogen Value in Cereals with Equipotential Interval- Potentiometric Titration Method

作　　者：刘晓庚[1] 李博[1] 苏才英[1] 陈梅梅[1] 邱顺顺[1] 张琪琪[1] 杜迎吉 黄磊[1] 杨婧雯[1] 鲍雯钰 

机构地区：[1]南京财经大学食品科学与工程学院/江苏省现代粮食流通与安全协同创新中心/江苏高校粮油质量安全控制及深加工重点实验室,江苏南京210023

出　　处：《粮食科技与经济》2016年第4期27-30,共4页Food Science And Technology And Economy

基　　金：江苏高校品牌专业建设工程项目(PPZY2015B52);南京财经大学重点建设课程项目(2014B22);江苏省高等学校大学生实践创新训练计划项目(201610327059Y)

摘　　要：探讨建立等电位间隔-电位微量滴定法连续测定谷物中脂肪酸值和氨基酸态氮值的方法。试验结果得到该法对谷物脂肪酸值和氨基酸态氮值测定的平均回收率分别为98.5%和99.8%,日内RSD分别为0.6%~1.6%和0.8%~2.2%,日间RSD分别为1.8%和4.6%,测定务必日内完成,检出限分别为0.12 mg KOH/100g和0.16 mg N/100g,测定准确性较国标法更优。应用效果表明,本法具有不必每个样品的每次测定都进行繁琐操作工作的优点,且样品和试剂等测定耗材消耗量也显著减少,测定快速,节时省工,大大地降低了测定成本,效果令人满意。The study is aimed to establish a equipotential interval-potentiometric titration method used for the determination of fatty acid and amino acid nitrogen value in cereals.The result was that the average recoveries of the method,to the determination of fatty acid and amino acid nitrogen value,were 98.5%and 99.8%respectively.The intra-day RSD were 0.6%~1.6%and 0.8%~0.6%respectively,while the inter-day RSD were 1.8%and 4.6%respectively.The determination had to be completed within one day and the detection limits were 0.12 mgKOH/100g and 0.16 mgN/100g respectively.The accuracy of this method was better than that of the national standard method.

关 键 词：等电位间隔-电位滴定法 连续测定 谷物 脂肪酸值 氨基酸态氮值 

分 类 号：O657.1[理学—分析化学] TS210.7[理学—化学]