叠氮溴化丙锭(PMA)在食源性致病菌检测中的应用  

Application of Propidium Monoazide(PMA) for Detection of Foodborne Pathogens

作　　者：肖莉莉[1] 张昭寰 娄阳[1] 孙晓红[1,2,3] 潘迎捷[1,2,3] 赵勇[1,2,3] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [3]上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《中国食品学报》2016年第6期187-194,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271870);上海市科学技术委员会部分地方院校能力建设项目(11310501100);上海市科学技术委员会科技创新行动计划项目(12391901300);上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号);上海市曙光计划项目(15SG48)

摘　　要：食源性致病菌是危害食品安全和人类健康的主要因素,对食源性致病菌进行检测是控制食源性疾病的关键环节。传统检测技术无法检测活的非可培养状态(VBNC)的细菌。基于核酸的检测方法克服了这一缺陷,然而难以区分"死菌"与"活菌"。叠氮溴化丙锭(PMA)为一种对死菌核酸具有高度结合能力的光敏染料,近年来,将其与核酸检测技术相结合,广泛应于多种食源性致病菌的活菌检测。本文综述了PMA的作用机理,影响PMA活菌检测的因素以及PMA在常见食源性致病菌检测中的应用,并预期PMA的进一步应用与后续研究情况。Foodborne pathogens are the main factor of food safety and human health, and detection of foodborne pathogens is the key element to control foodborne illness. Conventional culture methods could not detect viable but nonculturable（VBNC） cells, DNA-based molecular detection methods could overcome this drawback, and however these methods could not discriminate between viable and dead cells. In recent years, as a high affinity photoreactive DNA binding dye, propidiummonoazide in combination with DNA-based methods has been widely used to detect a variety of viable foodborne pathogens. The function mechanism of PMA, the factors for PMA to detectviable cells, and the application of PMA for detection of common foodborne pathogens were reviewed in this paper, and looked forward to the future application and researchof PMA.

关 键 词：食源性致病菌 叠氮溴化丙锭 活菌检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]