诃子有效部位在体外模拟体内消化道环境抗氧化活性变化  

Variation of antioxidant activities of the effective fraction from Terminalia chebula Retz in simulated in vivo digestive tracts

作　　者：夏德敏[1] 江浩[1,2] 江慎华[1,2] 谢丽琴[1] 金洪光[1] 张良慧[1,2] 沈勇根[1,3] 张爱琳[1,4] 

机构地区：[1]九江学院药学与生命科学学院,江西九江332000 [2]九江安德和生物科技有限公司,江西九江332005 [3]江西农业大学食品科学与工程学院,江西省天然产物与功能食品重点实验室,江西南昌330045 [4]天津农学院食品科学与生物工程学院,天津300384

出　　处：《食品工业科技》2016年第17期326-331,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31360371,31560308);江西省自然科学基金(20132BAB204030);江西省科技支撑计划(20123BBF60150、20151BBF60026);江西省卫生厅科研计划(2013A017);江苏省农产品物理加工重点实验室开放课题(JAPP2010-5);江西省天然产物与功能食品重点实验室开放基金资助项目;九江市科技支撑计划(201438);九江学院教学改革研究课题(2015-04)

摘　　要：为了研究诃子有效部位在体外模拟体内主要消化道环境中抗氧化活性的变化趋势与规律,本文对诃子有效部位经体外模拟体内主要消化道环境(口腔、胃、小肠)中处理后抗氧化活性(总多酚、总还原力、FRAP法抗氧化能力、DPPH自由基清除能力及总抗氧化能力)的变化进行了研究。结果表明,经人工模拟口腔环境处理后,诃子有效部位抗氧化活性显著降低(总多酚、总还原力、FRAP法抗氧化能力和DPPH自由基清除能力p<0.01,总抗氧化能力p<0.05);经人工模拟胃环境处理后,其抗氧化活性显著提高(p<0.01);经人工模拟小肠环境处理后,其抗氧化活性显著降低(总多酚、总还原力、FRAP法抗氧化能力和DPPH自由基清除能力p<0.01,总抗氧化能力p<0.05)。本文为后续在体内环境进行功能食品实验提供参考。In order to study the antioxidant variation of the effective fraction from TCR in simulated in vivo digestive tract environment,the effective fraction from TCR were immersed by conditions of mainly digestive tract（ mouth, stomach and small intestine）. The antioxidant activities of the effective fraction from TCR were studied by measuring total polyphenols, total reducing power, ferric reducing antioxidant power, free radical scavenging activity of DPPH and total antioxidant.The results indicated that antioxidant activities of the effective fraction of TCR were significantly reduced after immersed by artificial saliva （total polyphenols, total reducing power, ferric reducing antioxidant power,free radical scavenging activity of DPPH p 〈 0.01, total antioxidant p 〈 0.05）.While its antioxidant capacities were conspicuously increased after by artificial gastric juice （ p 〈 0.01 ）. Last, its antioxidant capacities were remarkably declined after immersed by artificial intestinal juice （total polyphenols, total reducing power,ferric reducing antioxidant power,free radical scavenging activity of DPPH p 〈 0.01 ,total antioxidant p 〈 0.05）.This study offered the reference for further research of functional food in vivo.

关 键 词：诃子 有效部位 人工唾液 人工胃液 人工肠液 抗氧化活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]