ε-聚赖氨酸高产菌选育与发酵性能评价  

Breeding and fermentation performance of a high-yield ε-poly-L-lysine producing strain

作　　者：郑根成[1] 王靓[1] 高阳[1] 向金鑫 陈旭升[1] 毛忠贵[1] 

机构地区：[1]江南大学生物工程学院,工业生物教育部重点实验室,江苏无锡214122

出　　处：《微生物学报》2016年第9期1450-1458,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31301556);中央高校基本科研业务费专项资金(JUSRP51504)~~

摘　　要：【目的】选育ε-聚赖氨酸(ε-PL)高产菌,并探究不同碳源对其发酵性能的影响。【方法】借助基因组重排和核糖体工程两种育种手段强化ε-PL产生菌的合成能力,并利用p H冲击工艺评价不同碳源对ε-PL发酵的影响。【结果】经过4轮基因组重排和4轮核糖体工程连续选育,获得1株高产突变株Streptomyces albulus GS114,其摇瓶ε-PL产量达到3.0 g/L,较出发菌提高了1.7倍。该改造菌株在5 L发酵罐中分别以葡萄糖和甘油为碳源进行192 h的补料-分批发酵时,ε-PL发酵产量分别达到了43.4 g/L和45.7 g/L,较出发菌提高了11.0%和14.9%,而菌体量分别减少了24.0%和33.2%,ε-PL得率提高了34.2%和30.7%。【结论】基因组重排结合核糖体工程育种是一种有效的ε-PL高产菌选育手段,研究结果将为ε-PL高产菌改造和工业生产碳源选择提供直接指导。[Objective] A high yield of ε-poly-L-lysine （ε-PL） producing strain was bred, and the effect of different carbon sources on fermentation performance was studied. [Methods] Genome shuffling and ribosome engineering were used to enhanced strain＇s productivity, and pH shock strategy was used to fermentation using different carbon sources. [Results] After four rounds of genome shuffling and ribosome engineering, we obtained a high yield mutant Streptomyces albulus GS 114 with ε-PL productivity of 3.0 g/L, which was 1.7 folds than that of the initial strain. When we performed fed-batch fermentation using glucose and glycerol as carbon sources in a 5-L fermenter, ε-PL productions reached 43.4 and 45.7 g/L after 192 h fed-batch fermentations, which were increased by 11.0 % and 14.9 % than that of Streptomyces albulus M-Z18, respectively. Meanwhile, the dry cell weights decreased by 24.0% and 33.2%, and ε-PL yields increased by 34.2% and 30.7%, respectively. [Conclusion] Genome shuffling and ribosome engineering are effective to breed high yield strains.

关 键 词：Ε-聚赖氨酸 链霉菌 基因组重排 核糖体工程 聚合度 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]