婴儿配方乳粉原辅料中细菌的分离鉴定  

Isolation and identification of bacteria from raw materials of powdered infant formula

作　　者：娄彬彬[1] 郭鸰[1] 周彦宏[1] 冯婧[1] 孙露宏 李明雨[1] 牛婕婷 费鹏[1] 满朝新[2] 姜毓君[1,2] 

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《中国乳品工业》2016年第8期9-11,31,共4页China Dairy Industry

基　　金：国家科技支撑计划课题(2013BAD18B11);黑龙江省杰出青年科学基金(JC201415);黑龙江省科研机构创新能力提升专项计划项(YC13D005);东北农业大学研究生科技创新基金(yjscx14060)

摘　　要：利用传统可培养方法对采集自婴儿配方乳粉加工厂的14类、42份原辅料样品进行细菌的分离,并采用16S rDNA基因测序技术对分离的细菌进行分子鉴定。结果表明:乳糖、氯化钾、维生素、低聚果糖、棕榈油和乳铁蛋白共6类原辅料中没有发现细菌,其他8类原辅料中共分离出36株细菌,包含了6个属10个种。原料乳和乳清粉是主要的细菌分离来源,共分离到11株和8株细菌,分别占总分离菌株的30.6%和22.2%。肺炎克雷伯菌和格氏乳球菌是优势菌株,共占总分离菌株的52.7%。In this study, bacteria were isolated by traditional cultivation-based method from 14 categories, 42 samples of raw materials collect- ed from powdered infant formula factory, and then identified the bacterial isolates by 16S rDNA sequence analysis. The results showed that bacteria were not found from the 6 kinds of raw materials, including lactose, potassium chloride, vitamin, fructo oligosaccharide, palm oil and lactoferrin. A total of 36 isolates were isolated from the other 13 different raw materials, and aU the isolates were classified into 6 genera and 10 species. Raw milk and whey powder were the main sources of isolated bacteria, and 11 and 8 bacteria were collected, accounting for 30.6% and 22.2% of the total isolates, respectively. Klebsiella pneumonia and Lactococcus garvieae were considered as the most prominent genera with a relative proportion of 52.7% of the total isolates.

关 键 词：婴儿配方乳粉 原辅料 细菌 分离鉴定 16S rDNA分析 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程]