No synergism between bis(propyl)-cognitin and rasagiline on protecting dopaminergic neurons in Parkinson's disease mice  

作　　者：Cheng-you Zheng Bao-jian Guo Wei Cai Wei Cui Shing-hung Mak Yu-qiang Wang Simon Ming-yuen Lee Yi-fan Han Zai-jun Zhang 

机构地区：[1]Institute of New Drug Research and Guangzhou Key Laboratory of Innovative Chemical Drug Research in Cardiocerebrovascular Diseases,Jinan University College of Pharmacy [2]School of Medicine,Ningbo University [3]Department of Applied Biology and Chemical Technology,Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University [4]State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences,University of Macao

出　　处：《Neural Regeneration Research》2016年第8期1339-1346,共8页中国神经再生研究（英文版）

基　　金：supported by grants from the Natural Science Foundation of Guangdong Province of China,No.2015A030313317;a grant from the Science and Technology Program of Guangzhou City of China,No.2014J4100097;partially by a grant from the Science and Technology Development Fund(FDCT) of Macao Special Administrative Region,No.134/2014/A3;a grant from the Research Committee of University of Macao,No.MYRG139(Y1-L4)-ICMS12-LMY and MYRG2015-00214-ICMS-QRCM;grants from the Research Grants Council of Hong Kong Special Administrative Region of China,No.561011,15101014;the Hong Kong Polytechnic University of China,No.G-SB10,G-UC15 and G-YBGQ

摘　　要：Rasagiline,a monoamine oxidase-B inhibitor,and bis（propyl）-cognitin（B3C）,a novel dimer are reported to be neuroprotective.Herein,the synergistical neuroprotection produced by rasagiline and B3 C was investigated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced mice of Parkinsonism.By using neurobehavioural tests,high-performance liquid chromatography and western blot assay,we showed that B3 C at 0.3 mg/kg,rasagiline at 0.02 mg/kg,as well as co-treatment with B3 C and rasagiline prevented MPTP-induced behavioural abnormities,increased the concentrations of dopamine and its metabolites in the striatum,and up-regulated the expression of tyrosine hydroxylase in the substantia nigra.However,the neuroprotective effects of co-treatment were not significantly improved when compared with those of B3 C or rasagiline alone.Collectively,we have demonstrated that B3 C at 0.3 mg/kg and rasagline at 0.02 mg/kg could not produce synergistic neuroprotective effects.Rasagiline,a monoamine oxidase-B inhibitor,and bis（propyl）-cognitin（B3C）,a novel dimer are reported to be neuroprotective.Herein,the synergistical neuroprotection produced by rasagiline and B3 C was investigated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced mice of Parkinsonism.By using neurobehavioural tests,high-performance liquid chromatography and western blot assay,we showed that B3 C at 0.3 mg/kg,rasagiline at 0.02 mg/kg,as well as co-treatment with B3 C and rasagiline prevented MPTP-induced behavioural abnormities,increased the concentrations of dopamine and its metabolites in the striatum,and up-regulated the expression of tyrosine hydroxylase in the substantia nigra.However,the neuroprotective effects of co-treatment were not significantly improved when compared with those of B3 C or rasagiline alone.Collectively,we have demonstrated that B3 C at 0.3 mg/kg and rasagline at 0.02 mg/kg could not produce synergistic neuroprotective effects.

关 键 词：nerve regeneration Parkinson＇s disease bis（propyl）-cognitin RASAGILINE monoamine oxidase B DOPAMINE multitarget synergism NEUROPROTECTION neural regeneration 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]