卵白蛋白-油酸复合物对人结肠癌细胞的HAMLET样生长抑制作用研究  

HAMLET- like cell- growth- inhibitory effect of ovalbumin- oleic acid complex on colon cancer cell

作　　者：邢效娟 刘建垒[1] 景浩[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品工业科技》2016年第18期133-138,142,共7页Science and Technology of Food Industry

基　　金："十二五"农村领域国家科技计划课题子课题(2012BAD28B08)

摘　　要：乳白蛋白与油酸形成的复合物具有癌细胞生长抑制作用（Human alpha-lactalbumin made lethal to tumor cells,HAMLET）。本文制备了卵白蛋白（Ovalbumin,OVA）和油酸（Oleic acid,OA）的复合物（OVA-OA）,通过检测细胞生长抑制、细胞膜通透性、细胞生长周期和凋亡的变化,分析了OVA-OA对人结肠癌细胞（Caco-2）的生长抑制作用以及其中OA对生长抑制作用的贡献。结果表明,当OVA-OA中OA的浓度达到182μmol/L时,OVA-OA对Caco-2细胞有显著（p〈0.05）生长抑制作用,273μmol/L时生长抑制作用增强,且随着作用时间的延长（1～3 h）而增强。在182μmol/L时,OA对Caco-2细胞也有生长抑制作用,其剂量效应关系与OVA-OA一致。但当OA浓度为273μmol/L时,其生长抑制作用弱于OVA-OA的。OVA-OA和OA均会促使乳酸脱氢酶（Lactate dehydrogenase,LDH）释放,增大晚期凋亡和坏死的细胞百分比,阻滞细胞周期在G0/G1期和S期。由此可知,OVA-OA对结肠癌细胞具有HAMLET样生长抑制作用,其中OA起主要作用。OVA-OA对人结肠癌细胞的生长抑制作用是通过损伤细胞膜、阻滞细胞生长周期和促进细胞凋亡来实现的。A complex of α-lactalbumin and oleic acid has previously been shown to have growth-inhibitory effect on cancer cells （Human alpha-lactalbumin made lethal to tumor cells, HAMLET）. Qvalbumin and oleic acid complex was prepared and its cell growth inhibition effect,along with contribution of oleic acid,were all evaluated on Caco-2 cell by using cell-growth inhibition,cellular membrane integrity,cell cycle and apoptosis assays.The result showed that OVA-OA significantly（ p 〈 0.05）inhibited cell growth at 182 μmol/L（ OA）, and the inhibitory effect was enhanced as the concentration was increased to 273 μmol/L.The inhibitory effect was also increased as incubation time was extended in a time-dependent manner. OA at 182 μmol/L also exhibited remarkable cell-growth- inhibitory effect in a similar pattern to OVA-OA,while its inhibitory effect was lower than that of OVA-OA at the same OA concentration of 273 μmol/L. Both OVA-OA and OA could increase LDH release rate, lead to cycle arrested at G0/G1 and S phases,and induce apoptosis.ln conclusion,the complex of OVA and QA has HAMLET- like cell-growth-inhibitory effect,which could be mainly contributed to OA.The mechanisms were associated with damage of cellular membrane integrity,arrest of cell cycle and induction of cell apoptosis.

关 键 词：卵白蛋白 油酸 细胞生长抑制 乳酸脱氢酶 细胞凋亡 细胞周期 

分 类 号：TS201.4[轻工技术与工程—食品科学]