臭氧降解黄曲霉毒素B_1污染玉米的体内毒性评价  

In vivo toxicity assessment of aflatoxin B_1-contaminated corn after ozone degradation

作　　者：罗小虎[1,2,3] 齐丽君[1,2,3] 王韧[1,2,3] 王莉[1,2,3] 李永富[1,2,3] 杨丹[1,2,3] 余晓斌[4] 陈正行[1,2,3] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]江南大学食品学院,江苏无锡214122 [4]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与机械》2016年第8期58-62,共5页Food and Machinery

基　　金：公益性行业(农业)科研专项项目(编号:201203037);公益性行业(粮食)科研专项项目(编号:201313005;201513006);国家自然科学基金(编号:31371874;31501579);国家国际科技合作专项(编号:2015DFA30540);江苏省博士后基金(编号:1501078B)

摘　　要：以黄曲霉毒素B1污染玉米(Aflatoxin B1-contaminated corn,ACC)为原料,评估经90 mg/L臭氧处理40 min的ACC(83.0μg/kg)在小鼠体内的毒性。结果表明,与基础饲料相比,ACC饲喂小鼠的平均体重显著降低(P<0.05),肝脏与肾脏体重比显著增加(P<0.05),血清中谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性显著升高(P<0.05),总蛋白、白蛋白和球蛋白含量显著降低(P<0.05)。而ACC经臭氧处理后饲喂小鼠,小鼠体重、饲料消耗量、肝脏与肾脏体重比均无显著差异,血清中谷丙转氨酶活性、总蛋白、白蛋白和球蛋白含量也无显著差异(P>0.05);而谷草转氨酶和碱性磷酸酶活性显著升高(P<0.05)。ACC可导致小鼠多项生理、生化指标发生显著变化,而ACC经臭氧处理后能显著改善其变化。Aflatoxin Bl contaminated corn （ACC） （83.0μg/kg） was treated for 40 min at 90 mg/L ozone. And mice were used to assess the vivo toxicity of ozone treated ACC. Results indicated that compared with basal feedstuff, the mean weight of mice fed with ACC significantly decreased （P〈0.05 ）. Whereas, the liver and kidney/body weight ratio increased significantly （P〈0.05 ）. Alanine t rans aminase （ ALT）, aspartate aminotransferase （ AST）, and alkaline phosphatase （ALP） in serum increased significantly （P〈0.05）. The total protein （TP）, albumin （ALB）, and globulin （GLB） content significantly decreased （P〈0.05）. When mice were fed with the o zone-treated ACC, no significant differences were observed in the mice mean weight, as well as the liver and kidney/body weight ratio. Additionally, no significant differences occurred in the major serum indexes ALT, TP, ALB, and GLB （P〉0.05）. Meanwhile, AST and AI,P increased significandy （P〈0.05）, The study indicated that ACC can lead to significant changes in various physiological characteristics and biochemical indexes in liver and kidney tissues, while the ozonetreated ACC could significantly improve these changes.

关 键 词：黄曲霉毒素B1 玉米 臭氧 降解 毒性 

分 类 号：R114[医药卫生—卫生毒理学]