三叶木通不同部位多酚、黄酮含量及抗氧化活性比较  

Comparative study on polyphenol,flavonoid and antioxidant activities from different parts of Akebia Trifoliata.

作　　者：陆俊[1,2] 罗丹[1] 张佳琦[1] 黄晖[1] 富春亚 李忠海[1,2] 

机构地区：[1]中南林业科技大学食品科学与工程学院,湖南长沙410004 [2]稻谷及副产物深加工国家工程实验室,湖南长沙410004

出　　处：《食品与机械》2016年第8期132-135,223,共5页Food and Machinery

基　　金：湖南省科技支撑计划项目(编号:2015NK3022)

摘　　要：分别用超声波法和回流提取法对三叶木通不同部位进行提取,测定不同处理条件下不同部位的多酚和黄酮含量及抗氧化能力。结果表明,超声波提取法优于回流提取法,不同部位中以藤茎超声波提取物多酚、黄酮含量最高和抗氧化能力最强,其多酚含量(TPC)、黄酮含量(TFC)、DPPH自由基清除能力(DPPH)、ABTS自由基清除能力(ABTS)、铁还原抗氧化能力(FRAP)值分别为(63.033±0.482)mg/g,(26.395±0.731)mg/g,(380.860±5.462)μmol Trolox/g,(337.181±1.586)μmol Trolox/g,(387.406±1.773)μmol Trolox/g。两种方法测得的多酚和黄酮含量及抗氧化活性均呈现以下趋势:藤茎>叶片>果皮>种子>果肉。Spearman相关性研究表明,多酚、黄酮含量与抗氧化活性呈强相关。研究结果为三叶木通的深加工利用提供了理论依据。In order to evaluate the content of polyphenol and fla- vonoid, as well as antioxidant activity in different parts of Akebia trifoliata, the ultrasonic-assisted and reflux method were used. The content of polyphenol and flavonoids, and antioxidant capacity in different parts were compared. The results showed that the ultrasonic extraction method was more efficient than the reflux extraction method, among the all extracts, the highest content of polyphenol and flavonoid, and strongest antioxidant capacity was found in cane extract by ultrasonic-assited （CSJ）. The contents of the total poly phenol, flavonoid in CSJ as （63. 033± 0. 482） mg/g, （26. 395±0.731） mg/g, respectively, and the DPPH, ABTS, FRAP antioxi dant activity value in CSJ as （380. 860± 5. 462）μmol Trolox/g, （337.181±1.586）μmol Trolox/g and （387.406±1.773） umol Trolox/g, respectively. The polyphenol, flavonoids content and antioxidant activity for all extracts were showing the following tendency： cane 2〉 leaf 2〉 peel 2〉 seed 〉 sarcoearp. Spearman correlation study shows that the content of polyphenol, flavonoids and slrongly related to the antioxidant activity. The research results provide experimental basis for deep processing of Akebia Trifoliata.

关 键 词：三叶木通 抗氧化 黄酮 多酚 

分 类 号：R284.1[医药卫生—中药学]