响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究  

Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase

作　　者：瞿叶辉 丁利君[1] 彭文媛 刘珊珊[1] 黄金[2] 成晓玲[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]广东工业大学材料与能源学院,广东广州510006

出　　处：《广东工业大学学报》2016年第5期77-82,共6页Journal of Guangdong University of Technology

基　　金：广东省教育部产学研合作重大专项(2013A090100009)

摘　　要：以罗非鱼加工鱼片的下脚料鱼头、鱼骨、鱼尾等为原料,选用弹性蛋白酶对其水解,通过响应面进行分析并优化酶解条件.以水解度与多肽含量为指标,分别对酶浓度、温度、p H值、料液比、酶解时间等因素对弹性蛋白酶酶解罗非鱼下脚料水解效果的影响进行分析.结果表明,弹性蛋白酶水解罗非鱼加工下脚料的最佳条件为:酶浓度0.553 mg/g,料液比1∶5、自然p H、温度41.94℃、酶解3.31 h,在此条件下得到的水解度为18.09%,多肽含量为0.9 mg/m L.The head, bones, and other raw waste of Tilapia are hydrolyzed by elastase, and response sur-face analysis method is used to optimize the enzymatic processes. The enzyme concentration, tempera-ture , pH , solid to liquid ratio, time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content. The results show that the optimum conditions of elastase hydrolysis： enzyme concentration 0. 553 mg/g, temperature 41. 94 ℃ , time 3. 31 h , pH natu-ral, solid-liquid ratio 1 ： 5, the degree of hydrolysis and the peptide content is separately 18. 0 9 % and 0. 90 mg /ml in this condition.

关 键 词：罗非鱼下脚料 响应面分析 弹性蛋白酶 水解度 多肽含量 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]