活性炭吸附分离玉米低聚肽中芳香族氨基酸的研究  

Study on Application of the Adsorption Aromatic Amino Acid of Active Carbon from Corn Oligopeptide

作　　者：王海轩[1] 谷瑞增[1] 张海欣[1] 金振涛[1] 李国明[1] 蔡木易[1] 

机构地区：[1]中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《食品工业》2016年第9期71-74,共4页The Food Industry

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102205-02);科技北京百名领军人才培养工程项目(Z131110000513026)

摘　　要：以玉米低聚肽为原料,采用静态吸附法,对8种活性炭进行了筛选,并探讨了吸附时间、活性炭与玉米低聚肽质量比和pH对玉米低聚肽F值的影响。在单因素试验的基础上,通过正交试验优化吸附工艺。结果表明,活性炭吸附工艺为吸附时间3.0 h,活性炭与玉米低聚肽质量比1.5︰1,pH 6.5。在此条件下制得的高F值低聚肽样品F值为32.77,凝胶过滤色谱测定其分子质量分布为连续的,绝大部分分布在132-576 u。这些数据为进一步开发高F值低聚肽提供了可靠的科学依据。Taking corn oligopeptide as raw material, the absorption of eight active carbon was investigated by the method of static absorption and the optimal active carbon was determined. The effects of time, m （C） ： m （peptide） ratio and pH on the Fischer ratio of corn oligopeptide were identified. Based on single factor test, an orthogonal design was applied to optimize adsorption process. The results showed that activated carbon adsorption conditions as follows： adsorption time 3.0 h, m （C） ： m （peptide） ratio=1.5 ： 1 and pH 6.5. Under these conditions, the Fischer ratio of the resulting high Fischer ratio oligopeptide sample was 32.77. G-25 gel filtration chromatography was used to determine the molecular weight of the oligopeptide. The molecular weight ranged within 132-576 u. The experimental results provided evidence for general development of high Fischer ratio oligopeptide.

关 键 词：活性炭吸附 芳香族氨基酸 高F值低聚肽 

分 类 号：TQ424.1[化学工程]