新、陈龙井茶关键香气成分的SPME/GC-MS/GC-O/OAV研究  

Investigations on the Aroma Active Compounds in Fresh and Aged Longjing Tea by SPME/GC-MS/GC-O/OAV

作　　者：舒畅[1] 佘远斌[1,2] 肖作兵[1] 徐路[2] 牛云蔚[1] 朱建才[1] 

机构地区：[1]上海应用技术学院香料香精技术与工程学院,上海201418 [2]绿色化学合成技术国家重点实验室培育基地,浙江工业大学化学工程学院,浙江310014

出　　处：《食品工业》2016年第9期279-285,共7页The Food Industry

基　　金：国家自然科学基金面上项目(21476270,21276006)

摘　　要：为探究新、陈龙井茶关键香气成分的不同,采用顶空固相微萃取(Head Space-solid Phase Microextraction,HS-SPME)、气相色谱-质谱(Gas Chromatography-mass Spectrometry,GC-MS)、气相色谱-嗅觉测量方法 (Gas Chromatography-olfactometry,GC-O)和香气活性值分析(Odor Activity Value,OAV)系统分析了新、陈龙井茶香气成分的特征与差异。经GC-MS在新龙井茶中鉴定出83种香气成分,在陈龙井茶中鉴定出84种香气成分,其中,反,反-2,4-庚二烯醛、6-甲基-2-庚酮是陈龙井茶特有的成分,己酸甲酯是新龙井茶特有的成分。联合GC-O和OAV在新龙井茶中鉴定出23种香气活性成分,在陈龙井茶中鉴定出26种香气活性成分。其中,β-紫罗兰酮、2-甲基丙醛、2-甲基丁醛、癸醛和己醛对新、陈龙井茶香气贡献较大。结果表明,联合GC-O和OAV不仅能有效地鉴定出新、陈龙井茶关键香气成分的差异,也为鉴别和评价茶叶品质提供了一种可靠的技术手段。The purpose of this study was to identify the key aroma compounds of longjing tea and develop a method to discriminate fresh and aged Longjing by analysis and characterization of the volatile aroma compounds.The aroma-active compounds in fresh and aged Longjing were characterized by gas chromatography -olfactometry/detection frequency analysis （GC-O/DFA） and odor activity value （OAV） analysis. By GC-MS analysis, 83 and 84 volatiles were identified from the fresh and aged Longjing Tea, respectively. 2-methylbutyraldehyde, isobutyraldehyde, 3-Methyl butanal and Methyl isobutenyl ketone were identified in appreciable abundance in both fresh and aged Longjing Tea. Trans, trans-2, 4-Heptadienal and 6-Methyl-2-heptanone were found only in the aged Longjing tea, while methyl acetate was found only in the fresh LonGing. By GC-O and OAV, 23 and 26 odor-active compounds were identified from the fresh and aged Longjing Tea, respectively. The results indicate that by combining GC-O and OAV techniques, the difference in the key aroma components between fresh and aged Longjing Tea could be effectively identified. The proposed method would provide a reliable tool for discrimination and quality evaluation of the fresh and aged Longjing Tea.

关 键 词：龙井茶 关键香气成分 香气活性值 气相色谱-嗅觉测量方法 

分 类 号：O657[理学—分析化学] TS272.7[理学—化学]