胶体金技术快速测定葡萄酒中赭曲霉毒素A  

Rapid detection of ochratoxin A in wines by gold immunochromatography assay

作　　者：庞世琦[1] 刘青[1] 李志勇[1] 梁瑞婷[1] 张子皓[1] PANG Shi-Qi LIU Qing LI Zhi-Yong LIANG Rui-Ting ZHANG Zi-Hao(Inspection and Quarantine Technology Center of Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangdong Key Laboratory of lmport and Export Technical Measures of Animal, Plant and Food, Guangzhou 510623, China)

机构地区：[1]广东出入境检验检疫局检验检疫技术中心广东省动植物与食品进出口技术措施研究重点实验室,广州510623

出　　处：《食品安全质量检测学报》2016年第8期3073-3076,共4页Journal of Food Safety and Quality

基　　金：国家质检总局科技项目((2016IK050);广东省科技厅国际合作项目(2013B051000068);广东省科技厅公益项目(2014A04041065)~~

摘　　要：目的利用胶体金免疫层析技术建立葡萄酒中赭曲霉毒素A的快速筛查方法。方法样品经过层析、孵育,通过i Check-单卡单测快检仪的图像分析进行定量检测,同时对进口与市售国产葡萄酒进行筛查。结果赭曲霉毒素A在添加水平为2.0、5.0μg/L时,红葡萄酒的平均回收率分别为92.5%、72.0%;相对标准偏差(RSD,n=6)分别为5.84%、2.59%;桃红葡萄酒的平均回收率分别为87.0%、76.6%;相对标准偏差(RSD,n=6)分别为7.70%、5.56%;白葡萄酒的平均回收率分别为84.0%、78.4%;相对标准偏差(RSD,n=6)分别为5.67%、3.54%。该方法定量限为1.0μg/L。同时与酶联免疫方法和液相色谱方法比较,通过t检验表明胶体金法不存在系统误差。市售葡萄酒赭曲霉毒素A的含量低于欧盟的限量标准(2.0μg/L)。结论胶体金的方法具有快捷、灵敏、特异性等特点,适合于大批量葡萄酒样品的现场快速筛查。Objective To establish a rapid method for the detection of ochratoxin A（OTA） in wines by gold immunochromatography assay（GICA）. Methods After chromatography and incubation, the samples were quantitatively detected by i Check-single card single measuring detector, meanwhile some of imported and domestic wines were screened. Results The recoveries of OTA spiked at 2 levels of 2.0 and 5.0 μg /L in red wine were 92.5% and 72.0% respectively with the relative standard deviations（RSDs） of 5.84% and 2.59%（n=6）; in pink wine were 87.0% and 76.6% respectively with the RSDs of 7.70% and 5.56%（n=6）; and in white wine were 84.0% and 78.4% respectively with the RSDs of 5.67% and 3.54%（n=6）. The quantification limit of GICA was 1.0 μg /L. Compared with enzyme-linked immunosorbent assay（ELISA） and high performance liquid chromatography（HPLC）, the results of t-test demonstrated that there was no systematic error in GICA. The results of market wines met the requirements of European Union limit standard（2.0 μg /L）. Conclusion The GICA method is rapid, sensitive and specific, which is suitable for preliminarily on-site screening of OTA in the samples of great batch rapidly.

关 键 词：葡萄酒 赭曲霉毒素A 胶体金免疫层析技术 快速筛查 

分 类 号：O658.1[理学—分析化学] TS262.6[理学—化学]