单宁酶产生菌的筛选及其固态培养基的优化  

Screening of tannase-producing strains and optimization of solid medium

作　　者：李浩洋[1] 付博宇 谷新晰[1] 李晨[1] 鄢梦洁 杜再慧 卢海强[1] 田洪涛[1] 

机构地区：[1]河北农业大学食品科技学院,保定071001

出　　处：《食品科技》2016年第9期8-13,共6页Food Science and Technology

基　　金：河北省高等学校科学技术研究项目(BJ2014036)

摘　　要：从柿子果园土样中分离获得一株单宁酶产生菌,经菌落形态观察、ITS区域序列同源性分析最终鉴定该菌为黑曲霉,命名为Aspergillus niger NHF5。利用响应面分析法对其产酶培养基进行优化,根据Box-Benhnken中心组合设计原理,对固态培养基中的3种主要成分:碳源、氮源和诱导物进行优化,最终确定3因素的最佳种类及添加量分别为:麦麸57.6%、豆粕38.4%、柿渣4.0%,此时粗酶液酶活可提高3.98倍,且该酶在柿子果汁脱涩方面具有显著作用。A tannase-producing strain isolated and screened from soil of persimmon orchards, it was identified as Aspergillus niger based on the observation of its colony morphology and the sequence analysis of ITS region, named Aspergillus niger NHF5. The medium for tannase produce of the strain was optimized by response surface analysis, according to Box-Benhnken central composite design principle, three main components-carbon, nitrogen, and inducer in the solid medium were optimized.The results showed that the amount of the best carbon source was wheat bran 57.6%, the amount of the best nitrogen source was the soybean meal 38.4%, the amount of the best inducer was persimmon slag 4.0%, the produced tannase activity can improve 3.98 times, the enzyme has a significant role in terms of persimmon juice deastringency.

关 键 词：黑曲霉 单宁酶 响应面 培养基 

分 类 号：TS201.3[轻工技术与工程—食品科学]