体外消化对玉米谷蛋白酶解物抗氧化活性的影响  

Effect of in vitro Digestion on the Antioxidant Activities of Hydrolysate from Corn Glutelin

作　　者：许瑞雪[1] 刘晓兰[1] 王俊彤[1] 郑喜群[1] XU Rui-xue LIU Xiao-lan WANG Jun-tong ZHENG Xi-qun(College of Food and Biotechnology, Qiqihar University/Key Laboratory of Processing Agricultural Products of Heilongjiang Province Normal University, Qiqihar 161006, Heilongjiang, China)

机构地区：[1]齐齐哈尔大学食品与生物工程学院/农产品加工黑龙江省普通高校重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品研究与开发》2016年第16期1-5,共5页Food Research and Development

基　　金：国家自然科学基金资助项目(31371726);黑龙江省自然科学基金重点资助项目(ZD2016005);黑龙江省高校科技成果产业化前期研发培育项目资助计划(1252CGZH05);齐齐哈尔市科学技术局资助项目(GYGG-201208);齐齐哈尔大学研究生创新科研项目(YJSCX2015-025X)

摘　　要：以玉米蛋白粉为原料提取玉米谷蛋白,利用蛋白酶对其进行酶解,制备蛋白水解物,再用胃蛋白酶及胰蛋白酶对其进一步消化处理,以抗氧化活性为指标对消化过程中蛋白酶加量进行优化,制备富含谷氨酰胺的具有抗氧化功能的蛋白水解物。结果表明,玉米谷蛋白经复合蛋白酶水解后,蛋白收率(54.41±3.26)%,水解物的羟基自由基清除率、亚铁离子螯合能力及还原力分别达到(29.50±0.31)%、(73.33±0.62)%及0.50±0.01;二步消化后蛋白水解物羟基自由基清除率、亚铁离子螯合能力及还原力分别达到(55.88±0.94)%、(90.44±0.01)%及0.40±0.01。In present paper,the corn glutelin was extracted from corn gluten meal （CGM）, and then it was hy-drolyzed by protamax. Further,the hydrolysate was digested by pepsin and trypsin,the concentrations of pepsin and trypsin were optimized when antioxidant activities of the hydrolysates as an evaluation criteria for the prepa-ration of hydrolysate,which were rich in Gln. The results showed that the yield of hydrolysate by protamex was （54.41±3.26）%,and its hydroxy radical scavenging capacity,ferrous ion chelating ability and reducing power were（29.50±0.31）%,（73.33±0.62）%and 0.50±0.01,respectively. The hydroxy radical scavenging capacity, ferrous ion chelating ability and reducing power of the hydrolysates after the digestion were （55.88±0.94）%, （90.44±0.01）%and 0.40±0.01,respectively.

关 键 词：玉米谷蛋白 消化 胃蛋白酶 胰蛋白酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]