原桃胶中1株芽孢杆菌的分离鉴定及其主要抗菌物质  

Isolation and Identification of a Bacillus from Peach Tree Gum and Its Main Antimicrobial Substance

作　　者：贾锐[1] 陆兆新[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2016年第21期136-143,共8页Food Science

基　　金：国家自然科学基金面上项目(31271936)

摘　　要：从天然原桃胶分离到1株产抑菌活性物质的芽孢杆菌TJ12。其发酵产物对金黄色葡萄球菌(Staphylococcus aureus)、藤黄微球菌(Micrococcus luteus)、蜡样芽孢杆菌(Bacillus cereus)和串珠镰刀菌(Fusarium moniliforme)有抑菌活性。结合菌落形态、生理生化指标、16S rRNA序列和gyr B序列将TJ12菌株鉴定为地衣芽孢杆菌(Bacillus licheniformis)。通过硫铵沉淀、有机溶剂萃取、羟丙基葡聚糖凝胶、高效液相色谱分离纯化,得到其主要活性物质。该物质对pH值、温度和3种蛋白酶(胰蛋白酶、蛋白酶K、胃蛋白酶)有较好的稳定性。经电喷雾电离质谱分析,初步鉴定该株地衣芽孢杆菌TJ12的主要抗菌物质为杆菌肽A。A strain of Bacillus named as TJ12 was isolated from peach tree gum,which could produce antibacterial substances.Its fermentation products had antibacterial activity against Staphylococcus aureus,Micrococcus luteus,Bacillus cereus and Fusarium moniliforme.Through colony morphology,physiological and biochemical characterization and 16 S rRNA and gyrB sequence analysis,the strain was identified as Bacillus licheniformis.The major bioactive substance was separated from its fermentation supernatant and purified by ammonium sulfate precipitation,organic solvent extraction,Sephadex LH-20 column chromatography and high performance liquid chromatography.The major bioactive substance had strong resistance to pH,temperature,and three proteases（trypsin,proteinase K,and pepsin）.By electrospray ionization-mass spectrometry analysis,we preliminarily identified this substance as bacitracin A.

关 键 词：原桃胶 地衣芽孢杆菌 抗菌物质 分离纯化 鉴定 杆菌肽A 

分 类 号：TS201.3[轻工技术与工程—食品科学]