未折叠蛋白反应相关因子葡萄糖调节蛋白78、X-盒结合蛋白1在食管癌中的表达及其意义  

Expression of unfolded protein response gene glucose regulated protein 78 and X-box binding protein 1 in esophageal squamous cell carcinoma and their clinical significance

作　　者：赵和平[1] 郝轶群[2] 张娜[1] 申凤俊[1] 许翠萍[1] 郭俊芝[1] Zhao Heping Hao Yiqun Zhang Na Shen Fengjun Xu Cuiping Guo Junzhi(Department of Gastroenterology, the First Hospital of Shanxi Medical University, Taiyuan 030001, China Department of Rheumatology, the First Hospital of Shanxi Medical University, Taiyuan 030001, China)

机构地区：[1]山西医科大学第一医院消化内科,太原030001 [2]山西医科大学第一医院风湿免疫科,太原030001

出　　处：《肿瘤研究与临床》2016年第10期683-686,共4页Cancer Research and Clinic

摘　　要：目的：探讨未折叠蛋白反应相关因子葡萄糖调节蛋白78（GRP78）及X-盒结合蛋白1（XBP1）在食管鳞状细胞癌中的表达、其对内质网应激（ERS）的激活作用及其在食管癌浸润生长中的作用机制。方法通过免疫组织化学方法检测30例食管鳞状细胞癌及30例正常食管鳞状上皮组织中GRP78、XBP1的表达，分析其与患者预后的关系。结果食管鳞状细胞癌中GRP78阳性表达率为83.3%（25／30），食管正常鳞状上皮组织中为20.0%（6／30）（χ2＝25.833，P＜0.05）。食管鳞状细胞癌中XBP1阳性表达率为70.0%（21／30），食管正常鳞状上皮组织中为26.7%（8／30）（χ2＝20.872，P＜0.05）。肿瘤浸润肌层者的GRP78和XBP1阳性表达率明显高于浸润黏膜层者。结论食管鳞状细胞癌组织中GRP78和XBP1高表达，可能参与了食管鳞状细胞癌的发生、发展。Objective To investigate the expression of unfolded protein response （UPR） gene glucose regulated protein 78（GRP78） and X-box binding protein 1（XBP1） in esophageal squamous cell carcinoma, its effect on activation of the endoplasmic reticulum stress （ERS） and its mechanism in the growth of esophageal squamous cell carcinoma. Methods The expressions of GRP78 and XBP1 were detected by immunohistochemistry in 30 samples of esophageal squamous cell carcinoma and 30 samples of normal esophageal squamous epithelium. The correlation between expressions of both proteins and prognosis was analyzed. Results GRP78 positive rate was 83.3 %（25/30） in esophageal carcinoma, while the proportion was 20.0 %（6/30） in normal esophageal （χ2=25.833, P〈0.05）. XBP1 positive rate was 70.0 % （21/30） in esophageal carcinoma, while the proportion was 26.7%（8/30） in normal esophageal（χ2=20.872, P〈0.05）. The positive rates of GRP78 and XBP1 in invasive muscular layer of esophageal squamous cell carcinoma were significantly higher than those in invasive mucous layer. Conclusion GRP78 and XBP1 are highly expressed in esophageal squamous cell carcinoma, which may involve the occurrence and development of the esophageal carcinoma.

关 键 词：食管肿瘤 癌 鳞状细胞 未折叠蛋白反应 葡萄糖调节蛋白78 X-盒结合蛋白1 GLUCOSE regulated PROTEIN 78 X-BOX binding PROTEIN 1 

分 类 号：R735.1[医药卫生—肿瘤]