超声波细胞破碎-高效液相色谱法定性定量分析黄骨鱼皮肤中的着色剂  

Qualitative and quantitative analysis of pigments in the skin of Pelteobagrus fulvidraco by high performance liquid chromatography with ultrasonic cell-break

作　　者：张飞[1] 刘军[1] 王和平[1] 方军[1] 张志舟 付强[1] 蔡群娣 郑家概[1] 

机构地区：[1]中国广州分析测试中心广东省化学危害应急检测技术重点实验室,广州510070

出　　处：《分析试验室》2016年第11期1321-1325,共5页Chinese Journal of Analysis Laboratory

基　　金：广东省科技计划项目(2013B091604003;2015B090906023与2013B020501005)资助

摘　　要：建立了测定黄骨鱼皮肤中3种合成着色剂(柠檬黄、日落黄和喹啉黄)和3种天然着色剂(叶黄素、β-胡萝卜素和虾青素)的分析方法。样品采用超声波细胞破碎法进行提取,提取不同的着色剂选用不同的提取剂。以叶黄素含量为指标,通过单因素试验确定了超声波细胞破碎法的最佳提取条件:全程时间8 min,超声/间隙时间2s/3s,超声功率比55%,保护温度35℃。采用C18柱(4.6 mm×250 mm,5μm),流速为1.0 m L/min,检测波长为446nm,分离不同的着色剂选用不同的流动相。合成着色剂和天然着色剂分别在2.5~50 mg/L和1.6~32 mg/L范围内呈良好线性,相关系数均大于0.9995。回收率在95.8%~102.5%之间,方法检出限在0.3~1.0 mg/kg之间。用该方法测定了12批次黄骨鱼,均检出含有叶黄素,其余5种未检出。A new method for the determination of three kinds of artificial pigments （ tartrazine, sunset yellow and quinoline yellow） and three kinds of natural pigments （lutein, 13-carotene and astaxanthin ） in the skin of Pelteobagrus fulvidraco was firstly proposed. Samples were extracted by ultrasonic ceil-break method. Different extraction solvents were selected according to different pigments. Based on the content of lutein, the experimental conditions were optimized through the single-factor experiment： full work time 8 min, work/terral time 2s/3s, ultrasonic power ratio 55% and temperature 35~C. HPLC separation was conducted on a C1s column （4. 6 mm ~ 250 mm, 5 i^m） at a flow rate of 1.0 mL/min and the detection was carried out at a wavelength of 446 nm. Different mobile phases were selected according to the separation of different pigments. The linear ranges of the method for artificial pigments and natural pigments were 2. 5 - 50 mg/L and 1.6 - 32 mg/L respectively with correlation coefficients not less than 0. 9995. The recoveries of the pigments were between 95.8% and 102. 5% , and the detection limits of the pigments for this method were between 0. 30 and 1.0 mg/kg. The method was applied in the determination of 12 batches of Peheobagrus fulvidraco. The lutein was detected and none of the rest of five pigments was detected. This method is found to be convenient, efficient and accurate, and could meet the determination of related pigments in the skin of Peheobagrus fulvidraco.

关 键 词：着色剂 黄骨鱼 超声波细胞破碎法 高效液相色谱 

分 类 号：TS201.6[轻工技术与工程—食品科学]