原料乳中玉米赤霉烯酮胶体金免疫试纸条检测方法的建立  

Development on detection card for determination of Zearalenone in raw milk

作　　者：丁木 姜霞[1] 王蕊[1] 孙露宏 周文琦[1] 赵玥明 满朝新[2] 姜毓君[1,2] 

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]国家乳业工程技术研究中心,哈尔滨150028

出　　处：《中国乳品工业》2016年第11期59-61,共3页China Dairy Industry

基　　金：国家科技支撑计划课题(2013BAD18B11);黑龙江省杰出青年科学基金(JC201415);黑龙江省科研机构创新能力提升专项计划项目(YC13D005)

摘　　要：采用柠檬酸三钠法合成胶体金,与抗玉米赤霉烯酮单抗(ZEA-m Ab)制成抗体-胶体金标记物,包被于胶体金结合垫上。玉米赤霉烯酮半抗原和蛋白的偶联物(ZEA-BSA)和羊抗鼠Ig G分别喷涂于硝酸纤维素膜作为检测线(T线)和质控线(C线),建立原料乳中玉米赤霉烯酮(ZEA)的胶体金免疫试纸条检测法。结果显示,试纸条的灵敏度为30n g/m L,与其他毒素无交叉反应,10 min内即可肉眼观察结果。检测添加有玉米赤霉烯酮的原料乳样品,该试纸条的检测限为50 ng/m L。方法可用于原料乳中玉米赤霉烯酮的快速检测。Gold colloid nanoparticles were prepared with chloroauric acid and sodium Citrate. The colloidal gold nanoparticles labeled with zearalenone monoclonal antibody were coated on the conjugated pad. The artificial antigen ZEA-BSA and goat anti-mouse IgG were jetted on the nitrocellulose membrane, respectively, as test line and control line. The results showed the limit of detection was 30 ng/ml, and the whole test time was about 10 min. None cross activity were observed with other mycotoxins. The method based on the dipstick can be applied to rapid detection ofzearelenone residues in raw milk.

关 键 词：玉米赤霉烯酮 试纸条 原料乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]