氢化物发生-原子荧光光谱法测定食品中有机硒和无机硒  

Determination of organic selenium and inorganic selenium in food by hydride generation-atomic fluorescence spectrometry

作　　者：谢逸欣[1] 黄仕稳[1] 鞠鹤鹏[1] 陈丽璇[1] 吴晖[2] 曾婷[1] XIE Yi-xin HUANG Shi-wen JU He-peng CHEN Li-xuan WU Hui ZENG Ting(Center for Disease Control and Prevention in Guangzhou Military Region, Guangzhou 510507 College of Light Industry and Food Science, South China University of Technology Guangzhou 510640)

机构地区：[1]广州军区疾病预防控制中心,广州510507 [2]华南理工大学轻工与食品学院,广州510640

出　　处：《食品科技》2016年第11期262-265,共4页Food Science and Technology

摘　　要：目的：建立食品中有机硒含量和无机硒含量的测定方法。方法：50%盐酸提取食品中无机硒成分,通过硝酸-高氯酸混合酸体系进行消解,测定无机硒含量。有机硒含量通过总硒与无机硒含量差减法获得。结果：硒的检出限是0.0028 mg/kg,线性范围是（0～10）ng/mL。经回收实验与实际样品检测,总硒加标回收率为83.82%～101.46%,相对标准偏差（RSD）为0.75%～3.79%;无机硒加标回收率为97.95%～102.47%,相对标准偏差（RSD）为0.64%～4.10%。结论：该测定方法线性范围广、重复性好、检出限低,适合用于测定食品中有机硒及无机硒含量。Objective： To establish a method for the determination of organic selenium and inorganic selenium in food by hydride generation-atomic fluorescence spectrometry. Methods： Inorganic selenium was extracted with the concentration of 50% hydrochloric acid and digested by nitric acid-perchloric acid system. Determination of organic selenium was the difference between total selenium and inorganic selenium content. Results： Detection limit of Se was 0.0028 mg/kg. Linear range of this determination method was（0～10）ng/mL. Recovery rates of determination in total selenium and inorganic selenium were 83.82%～101.46% and 97.95%～102.47%. The relative standard deviations of determination in total selenium and inorganic selenium were 0.75%～3.79% and 0.64%～4.10%. Conclusion： The methods for the determination of organic selenium and inorganic selenium in food were appropriate because of the wide linear range, good reproducibility, and low detection limit.

关 键 词：氢化物发生-原子荧光光谱法 有机硒 无机硒 食品 

分 类 号：TS207[轻工技术与工程—食品科学]