顶空-气相色谱快速检测发酵乳中4种风味物质  

Rapid Determination of Four Volatile Flavor Compounds in Milk Fermented by Mixture Strains by Headspace Gas Chromatography

作　　者：赵允[1] 李雪芳[1] 吴平[1] 戴绚丽[1] 

机构地区：[1]杭州娃哈哈集团有限公司,杭州310018

出　　处：《食品工业》2016年第12期280-283,共4页The Food Industry

摘　　要：为考察不同组合发酵剂发酵乳的产香特性,建立了一种快速简单地测定发酵乳中乙醛、丙酮、乙酸乙酯和2,3-丁二酮4种挥发性风味物质的顶空-气相色谱法。以HP-INNOWAX毛细管色谱柱为分离柱,氢火焰离子化检测器检测,优化程序升温条件,考察了顶空平衡温度、平衡时间和加盐量等对测定的影响。在优化的条件下,4种挥发性风味物质在选择的浓度范围内线性关系良好（r均不小于0.999 1）,平均回收率为80.2%-105.6%（相对标准偏差均小于6.5%）,方法检出限为0.01-0.06 mg/L。该方法操作简单、准确度高、高效快捷,适用于组合发酵剂发酵乳中4种挥发性物质的同时检测。To investigate the characteristics of flavor-producing milk fermented by mixture strains, a rapid and simple method was developed for the determination of acetaldehyde, acetone, ethyl acetate and 2, 3-butanedione by headspace gas chromatography （HS-GC）. The HP-INNOWAX capillary column and flame ionization detector were used for the separation and detection of volatile flavor compounds. The chromatographic conditions were optimized. Three parameters for HS in terms of equilibrium temperature, equilibrium time and salt concentration were optimized. Under the optimized conditions, four volatile flavor compounds showed good linear relationships with good correlation coefficients that r was not less than 0.999 1 in the prescribed concentration range. The average recoveries were 80.2%-105.6% with the relative standard deviations （RSD） less than 6.5%. The limits of detection of the method were 0.01-0.06 mg/L. The method was simple, rapid, sensitive and accurate, and it was suitable for the simultaneous determination of four voltatile compounds in milk fermented by mixture strains.

关 键 词：顶空气相色谱法 挥发性风味物质 组合发酵剂发酵乳 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]