新生儿疾病筛查中滤纸干血片长期保存的稳定性观察  

The stability observation of dried blood spots after long-term storage in neonatal screening

作　　者：于璐[1] 马志军[1] 胡锦春[1] 简永建 芦丽红[1] 李艳霞[1] 刘伟[1] 王琳琳[1] YU Lu MA Zhi-jun HU Jin-chun et al(Capital Medical University Affiliated Beijing Obstetrics and Gynecology Hospital, Beijing, 100026, China)

机构地区：[1]首都医科大学附属北京妇产医院北京妇幼保健院遗传代谢实验室,北京100026

出　　处：《中国优生与遗传杂志》2016年第12期102-104,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨新生儿疾病筛查滤纸干血片长期保存后TSH和Phe的稳定性。方法对2007-2011年间4℃保存的500份新生儿足跟血滤纸干血片,分别用时间分辨荧光免疫分析法和荧光计数分析法,常规检测和延长洗脱时间检测TSH和Phe水平,与原始检测结果进行对比分析。结果 4℃保存血片的Phe浓度值,与2007-2011年间的原始测定值比较,差异无统计学意义,P>0.05;TSH常规方法检测新值与原始测定值比较,保存1-2年组,差异无统计学意义,P>0.05,保存3-5年组,差异有统计学意义,P<0.01,延时法重复检测3-5年组血片,检测值与原始值比较,其差异有统计学意义,P<0.01。结论4℃保存1-5年血片Phe检测值与保存时间、洗脱时间无明显关系,与原始值无明显差异;血片保存两年内,TSH检测值可以保持稳定,保存3年及以上,检测值与原始值差异显著,且延长洗脱时间不能使TSH检测值恢复至原始水平。Objective：To discuss the stability of TSH and Phe detected value of neonatal dried blood filter cards after longterm storage and the accuracy of the original sample review. Methods：We use 500 newborn dried blood filter cards stored in 4℃from 2007 to 2011.Detected the TSH and Phe concentration levels of all the dried blood spots（DBS）,with time-resoveled fluorescence immunoassay analysis method and fluorescence counting method respectively,conventional detected and prolong the elution time detected the DBS kept under different environment,compared with the original values,and analysis. Results：The Phe values of the DBS samples stored at 4℃ compared with the original measured values between 2007-2011,he results shows no significant difference,P〉0.05. The DBS stored in 4℃ within 1-2 years,the TSH values detected by conventional method compared with the original values,the results shows no significant difference,P〉0.05,and that stored for 3-5 years,the results shows significantly different,P〈0.01,and we use the time-delay method detected the DBS stored for 3-5 years,the results also shows significantly different. P〈0.01.The TSH values detected by the time-delay method also have significantly different with the original values,P〈0.01. Conclution：Dried blood filter cards stored at 4℃ within 1 to 5 years,the Phe detected values have no obvious relationship with the save time and the elution time,and also no significant differences with the original values.The DBS stored within 1-2 years,the TSH values were stable,and that stored of 3-5 years,the TSH values have significant differences with the original values,and the time-delay method can not make the TSH values restored to its original levels.

关 键 词：新生儿疾病筛查 滤纸干血片 甲状腺功能低下 苯丙酮尿症 

分 类 号：R722.1[医药卫生—儿科]