响应面法优化菠萝叶纤维多酚提取工艺  

Optimization of extraction process for polyphenols from pineapple leaves fiber by response surface methodology

作　　者：汪泽[1,2] 崔丽虹[2] 唐冰[1,3] 魏晓奕[2] 付调坤[2] 张劲[4] WANG Ze CUI Li-hong TANG Bing WEI Xiao-yi FU Tiao-kun ZHANG Jin(College of Food Science and Technology of Huazhong Agricultural University, Wuhan 430070, China Agriculture Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, China Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China Agrieuhural Machinery Research Institute, Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091, China)

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]中国热带农业科学院农产品加工研究所,广东湛江524001 [3]中国热带农业科学院,海南海口571101 [4]中国热带农业科学院农业机械研究所,广东湛江524091

出　　处：《食品工业科技》2017年第1期219-223,共5页Science and Technology of Food Industry

基　　金：广东省自然科学基金(2014A030307009);中国热带农业科学院中央级公益性科研院所基本科研业务费专项(1630062015016)

摘　　要：研究乙醇回流法提取菠萝叶纤维中总多酚,通过响应面法优化工艺条件。在单因素实验的基础上,采用响应面实验设计优化菠萝叶纤维多酚的提取工艺,建立了该工艺的二次多项数学模型,考察乙醇体积分数、料液比、提取温度、提取时间四个因素及其交互作用对菠萝叶纤维多酚提取率的影响。实验结果表明,曲面回归方程拟合性良好,其中乙醇体积分数与提取温度的交互作用显著,在乙醇体积分数59%、料液比为1∶23、提取温度71℃、提取时间118 min的条件下,验证优化工艺得到菠萝叶纤维中多酚提取率为(1.67±0.02)mg/g,与预测值1.6978 mg/g相近。The extraction technology of total polyphenols from pineapple leaf fiber were investigated by using ethanol reflux method.Based on the single factor experimental analysis, through the response surfac, extraction conditions was optimized by investigating the effect of ethanol concentration, solid-to-solvent ratio, extraction temperature, extraction time and their interaction on thepolyphenols extracts and the process of quadric polynomial mathematics model was established. Experiment results showed that regression equation fit well with experimental data and the interaction effect of ethanol concentration and extraction temperature was significant. The optimal of extraction conditions including ethanol concentration, solid- to- solvent ratio, extraction temperature, extraction time were 59%, 1：23 （g/mL）,71 ℃ and 118 min respectively. The content of polyphenols extracted from pineapple leaf fiber under the conditions was（ 1.67 ± 0.02） mg/g closing to predicted extraction rate 1.6978 mg/g.

关 键 词：菠萝叶纤维 总多酚 提取工艺 响应面法 

分 类 号：TS209[轻工技术与工程—食品科学]