北京市集贸市场生鲜猪肉肠球菌的耐药特征分析  

Antimicrobial susceptibility analysis of Enterococcus spp. collected from a raw pork free trade market in Beijing

作　　者：彭子欣[1] 张爽[2] 王伟 胡豫杰[1] 吕涵阳 张建中[3] 李凤琴[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]北京农学院食品科学与工程学院,北京102206 [3]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国食品卫生杂志》2016年第6期738-743,共6页Chinese Journal of Food Hygiene

基　　金：中国博士后科学基金资助项目(2016M590072)

摘　　要：目的了解北京市某大型集贸市场内部环境样品和零售生鲜猪肉中分离肠球菌的耐药性特征。方法采用肉汤稀释法测定86株肠球菌对10种临床常用抗生素的药物敏感性。结果 86株肠球菌对四环素、红霉素、高浓度链霉素、环丙沙星、高浓度庆大霉素和氯霉素的耐药率分别为67.44%(58/86)、54.65%(47/86)、38.37%(33/86)、26.74%(23/86)、23.26%(20/86)和20.93%(18/86),耐3种以上抗生素的多重耐药肠球菌有30株,占34.88%,耐药谱共有22种。所有肠球菌对氨苄西林、青霉素和万古霉素均敏感。耐药谱型分析发现,不同样品来源肠球菌的耐药谱差异较大。另外,本研究从国内食品中分离到对达托霉素耐药的肠球菌,此前我国未见相关报道。结论北京市集贸市场内部环境和零售生鲜猪肉中分离的肠球菌耐药严重,并呈现一定的耐药谱型差异,而且分离到达托霉素耐药株,应引起关注。Objective To evaluate the antimicrobial resistance of Enterococcus spp. using samples from the environment and retailed raw pork from free markets in Beijing. Methods The susceptibility of 86 isolated Enterococcus spp. to 10 antibiotics was determined by broth microdilution method according to CLSI（ Clinical and Laboratory Standards Institute）.Results In all 86 isolates tested,67. 44%（ 58 /86）,54. 65%（ 47 /86）,38. 37%（ 33 /86）,26. 74%（ 23 /86）,23. 26%（ 20 /86） and 20. 93%（ 18 /86） of Enterococcus spp. were resistant to tetracycline,erythromycin,ciprofloxacin,high-level streptomycin,high-level gentamicin,and chloramphenicol,respectively. It should be pointed out that 30（ 34. 88%） strains of Enterococcus spp. were resistant to more than 3 antibiotics,and 22 spectrums of antimicrobial susceptibility were observed. All Enterococcus spp. were susceptible to ampicillin,penicillin and vancomycin. According to the antibiotic profiles analysis,Enterococcus spp. isolated from different sources had significant difference on the antibiotic profiles. Moreover, daptomycin-resistant Enterococcus spp. from food-related samples was first reported in China.Conclusion The multi-drug resistance of Enterococcus spp. isolated from raw pork and environmental samples of the free markets was very serious,and their antibiotic profiles presented significant difference. It was the first time to isolate a daptomycin-resistant Enterococcus spp. from food. Therefore,close attention should be paid to drug resistance surveillance.

关 键 词：肠球菌 药敏试验 耐药性 生鲜猪肉 集贸市场 食源性致病菌 

分 类 号：R155.5[医药卫生—营养与食品卫生学]