黍子的抗氧化活性及其与功能活性成分的关系  

The antioxidant activity and its relationship with the functional components in proso millet

作　　者：郑楠楠[1,2] 綦文涛[2] 王春玲[1] 允婷婷 幺杨 任贵兴 

机构地区：[1]天津科技大学,天津300222 [2]国家粮食局科学研究院,北京100037 [3]中国科学研究院作物科学研究所,北京100081

出　　处：《食品与机械》2016年第12期137-141,共5页Food and Machinery

基　　金：粮食公益性行业科研专项(编号:201313006-5)

摘　　要：通过建立C57BL/6肥胖小鼠模型,评价了黍子对小鼠肝脏抗氧化性能的影响。并在分析比较其多酚和黄酮含量的基础上,利用抗氧化化学分析和细胞评价试验,研究了黍子抗氧化活性与其主要功能活性物质含量之间的关系。结果表明:高脂饮食削弱了小鼠肝脏的抗氧化性能,日粮中添加黍子后,高脂饮食小鼠肝脏的抗氧化能力得到了明显的改善。不同黍子品种间多酚和黄酮的含量差异较大。总多酚含量变异系数可达63.61%,变幅为0.78~3.44mg/g。总黄酮含量变异系数可达68.46%,变幅为0.89~10.17 mg/g。不同品种黍子提取物的总抗氧化能力指数(ORAC)和细胞抗氧化活性(CAA)均存在显著性差异,且黍子提取物的ORAC和CAA值与黄酮和多酚的总含量相关,均呈正向线性关系,相关系数R^2分别为0.811和0.565。The effects of proso millets on the antioxidant activity of liver were evaluated by the high-fat diet C57BL/6 mice model,and the relationship of antioxidants with the contents of main bioactive compounds in proso millets were also studied based on the chemical and cellular analyses of the polyphenols and flavonoids contents.The results showed that the high-fat diet reduced the hepatic antioxidant capacity of liver,while the addition of proso millets could improve it significantly with a high-fat diet.The contents of both polyphenols and flavonoids were significantly different among different breeds of proso millets.The variation coefficient of total polyphenol was 63.61%,ranging from 0.78 to 3.44 mg/g,while that of the total flavonoid content was 68.46%,ranging from 0.89 to 10.17mg/g.The Oxygen Radical Absorbance Capacity（ORAC）and cellular antioxidant activity（CAA）of different breeds of proso millets were found different.Moreover,both of the ORAC and CAA of proso millet were correlated with the total contents of polyphenols and flavonoids,shown a positive linear relationship,and the linear coefficients（R^2）were 0.81 and 0.57,respectively.

关 键 词：黍子 抗氧化活性 黄酮 多酚 线性相关性 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]