离子选择电极法测定谷氨酰胺酶活力研究  

Research on Glutaminase Activity Determination by Ion Electrode Method

作　　者：崔春[1] 徐明[1] 杨娟[1] 王炜[1] CUI Chun XU Ming YANG Juan WANG Wei(College of Food Science and Engineering,South China University of Technology, Guangzhou 510641, China)

机构地区：[1]华南理工大学食品科学与工程学院,广州510641

出　　处：《中国调味品》2017年第2期10-14,共5页China Condiment

摘　　要：通过确定温度、pH值、铵离子强度调节剂、Na+含量等对铵离子选择性电极响应值的影响,建立一种谷氨酰胺酶活力的测定方法。结果显示:利用铵离子选择电极法测定谷氨酰胺酶活力时,pH值保证在2~4之间,尽量避免Na+的存在,选用20%氯化锂(溶于pH 2.0的无机酸)溶液作为铵离子强度调节剂。此方法测定结果准确度、精密度和稳定性均良好,实用性强。用于测定商用蛋白酶的谷氨酰胺酶活力实验发现多数商用蛋白酶具有谷氨酰胺酶活力,其中博仕奥碱性蛋白酶的谷氨酰胺酶活力达到14.4U/g,结果表明:一般商品化酶制剂中本身含有谷氨酰胺酶或者蛋白酶具有谷氨酰胺酶活力,同时也验证了此方法的可行性。By ensuring influence of temperature, pH value, strength regulator of ammonium ion and Na^＋ content on the response value of ammonium ion selective electrode, the determination method of glutaminase activity is established. The results show that when temperature is consistent with the calibration temperature, pH value is guaranteed between 2~4, avoiding the presence of Na^＋, and selecting 20 % lithium chloride （soluble in inorganic acid with pH 2.0） solution as strength regulator of ammonium ion. The method has good accuracy, precision and stability. In addition, the glutaminase activity of the commercial proteases is determined. The results indicate that most of the commercial proteases have glutaminase activity, including the Boshiao alkaline protease which reaches 14.4 U/g. This results may be attributed to the general commercial enzyme preparations containing glutaminase or they have glutaminase activity. At the same time, it also verifies the feasibility of this method.

关 键 词：谷氨酰胺酶 铵离子 离子选择性电极 酶活力 

分 类 号：TS201.25[轻工技术与工程—食品科学]