胶原蛋白水解物中L-羟脯氨酸两种检测法对比分析  

The Comparative Analysis of Two Methods of Detection to L-Hydroxyproline in Collagen Hydrolysate

作　　者：王海轩[1] 谷瑞增[1] 金振涛[1] 李国明[1] 鲁军[1] 蔡木易[1] 

机构地区：[1]中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《食品研究与开发》2016年第22期119-122,共4页Food Research and Development

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102205-02);科技北京百名领军人才培养工程项目(Z131110000513026)

摘　　要：应用紫外分光光度计和氨基酸自动分析仪检测胶原蛋白水解物中L-羟脯氨酸含量,分析了两种方法的适用性。结果显示,紫外分光光度计法和氨基酸分析仪法在0～2.95 mg/L范围内,L-羟脯氨酸的浓度分别和吸光度、峰面积的线性良好,相关系数R2分别为0.999 3和0.999 8,RSD分别为0.96%和0.73%（n=6）。紫外分光光度计法样品处理简便且重复性好,适合基层大量检测使用;氨基酸分析仪法操作简便且不受其他氨基酸干扰,适合精密和研究检测使用。The content of L-Hydroxyproline（Hyp）in collagen hydrolysate was measured by An UV spectrophotometer and an amino acid auto-analyzer（AAA）respectively. And the applicability of the two methods was demonstrated. The results showed that the linear relationships between L-Hydroxyproline concentration and absorbance,peak area were both good in the range of 0-2.95 mg/L. R2 were 0.999 3 and 0.999 8 respectively.And RSD were 0.96 % and 0.73 %（n=6）respectively. The UV Spectrophotometry was suitable for basic level detection because the samples could be handled easily and had a good reproducibility. The automatic amino acid analyzer was suitable for precision and research because of the advantages of easy operation and not affected by other amino acids.

关 键 词：胶原蛋白水解物 L-羟脯氨酸 紫外分光光度计法 氨基酸自动分析仪法 

分 类 号：TS207.3[轻工技术与工程—食品科学]