牛奶中主要过敏原α(s1)-酪蛋白的生物信息学分析  

Bioinformatics analysis for α_(s1)-casein of main allergens in milk

作　　者：范安妮[1] 佘之蕴[1] 梁宇斌[1] 张娟[1] 周臣清 

机构地区：[1]广东产品质量监督检验研究院,国家食品质量监督检验中心(广东),佛山528300

出　　处：《食品安全质量检测学报》2016年第12期4740-4744,共5页Journal of Food Safety and Quality

基　　金：广东省质量技术监督局科技项目(2013PS02)~~

摘　　要：目的用生物信息学方法分析预测牛奶中主要过敏原α_（s1）-酪蛋白的结构与功能。方法从Genbank搜索α_（s1）-酪蛋白的氨基酸序列,采用多种生物信息学分析手段对该序列进行分析预测,包括信号肽、疏水性、跨膜区、活性位点、B细胞表位、二级结构和三级结构。结果α_（s1）-酪蛋白的1~16位是信号肽,无跨膜区,有3个糖基化位点、5个酪蛋白激酶磷酸化位点、3个蛋白激酶C磷酸化位点。IEDB数据库预测α_（s1）-酪蛋白的抗原区分别为氨基酸的16~29、45~50、52~54、56~70、74~75、77~86、94~104、142~152、173~179和185~210,同时预测构建了α_（s1）-酪蛋白的二级结构和三级结构。结论预测得到的α_（s1）-酪蛋白结构和功能信息,可为牛奶过敏原酪蛋白的致敏机制研究及检测方法建立提供信息基础。Objective Toanalyze and predict the structures and functions of main allergenαs1-casein in milk by bioinformatics methods.MethodsThe amino acid sequence ofαs1-casein was searched in Genbank. Its signal peptides, hydrophobicities, transmembrane domains, active site, B-cell epitopes, the secondary structures and tertiary structure were predicted and analyzed by bioinformatics methods.ResultsThe predicted signal peptide was in the 1~16 amino acid secquence. It had no transmembrane region, 3 glycosylation sites, 5 casein kinase phosphorylation sites and 3 protein kinase C phosphorylation sites in the protein. After prediction by IEDB, the antigen areas ofαs1-casein were in the amino acid sites of 16~29, 45~50, 52~54, 56~70, 74~75, 77~86, 94~104, 142~152, 173~179 and 185~210, respectively. Meanwhile, the secondary structure and tertiary structure were predicted and built. ConclusionThe predicated structures and function information ofαs1-casein can provide information base for the sensitization mechanism research and detection of casein allergens in milk.

关 键 词：过敏原 α(s1）-酪蛋白 生物信息学 牛奶 

分 类 号：TS252.5[轻工技术与工程—农产品加工及贮藏工程]