蚕蛹蛋白酶法水解工艺优化  

Technology Optimization of Enzymolysis on the Silkworm Chrysalis Protein

作　　者：吴文湧 郑辉[1] 陈露[1] 张润秋[1] 鄢又玉[1] 陈西喆 钟方旭[1] WU Wen-yong ZHENG Hui CHEN Lu ZHANG Run-qiu YAN You-yu CHEN Xi-zhe ZHONG Fang-xu(School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, Hubei, China Hubei Shennong Honey Bio-Tech. Co., Ltd., Shiyan 442000, Hubei, China)

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023 [2]湖北神农蜂语生物产业有限公司,湖北十堰442000

出　　处：《食品研究与开发》2017年第1期107-112,共6页Food Research and Development

摘　　要：以水解度和多肽得率为评价指标,研究了中性蛋白酶对脱脂蚕蛹蛋白的酶解工艺。采用单因素与响应面优化设计,得出最佳酶解工艺条件为:酶解温度56℃、酶解液p H 7.7、加酶量3.9%(质量分数)、料液比1∶12(g/m L)、酶解时间2 h。在此优化试验条件下,蚕蛹蛋白水解度和多肽得率的预测值分别为24.94%和72.16%,实际值则分别为24.22%及70.61%,与模型预测值吻合。此工艺可推广应用于蚕蛹蛋白多肽产业化生产的备选工艺。The degree of hydrolysis and peptide yield are taken as evaluation index and the technology of enzymatic hydrolysis by neutral protease on defatted silkworm chrysalis protein has been studied. And the single factor design and response surface optimization experiments had been adopted,as a result the optimum enzymatic hydrolysis condition has also been obtained ：temperature of enzymolysis was 56 ℃,the p H of enzymatic hydrolysate was 7.7,the amount of enzyme was 3.9 %（mass fraction）,liquid to solid ratio was 1 ∶ 12（g/m L）and the enzymatic time was 2 hours. Under such optimum condition,the theoretical DH of silkworm chrysalis protein and the yield of peptide was predicted to be 24.94 % and 72.16 %,however,the actual ones were 24.22 %and 70.61 % respectively,both of which are fit well and the process can be extended to be applied as the alternative technology to the silkworm chrysalis protein polypeptide in the industrialization production.

关 键 词：蚕蛹 中性蛋白酶 水解 多肽 响应面优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]