植物乳杆菌及其近缘种部分看家基因的系统发育分析  

Phylogenetic Analysis of Lactobacillus plantarum and Related Species Using Partial Housekeeping Genes

作　　者：乌日拉嘎[1] 徐海燕[1] 宋宇琴[1] 冯淑贞[1] 孙志宏[1] 斯琴巴特尔[2] 包英姝 张和平[1] 孟和毕力格[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018 [2]锡林郭勒盟蒙医研究所,内蒙古锡林浩特026000

出　　处：《现代食品科技》2017年第1期100-105,共6页Modern Food Science and Technology

基　　金：内蒙古科技重大专项(20140125);中国农业研究体系(CARS-37)

摘　　要：本文研究了16S rRNA基因、pheS和pyr G部分基因序列对Lactobacillus plantarum(L.plantarum)及其近缘种的区分能力,采用分离自酸面团、酸牛奶和泡菜中的10株L.plantarum为研究对象,以其看家基因pheS和pyr G部分基因序列作为分子标记,结合已上传至Gene Bank的近缘种的相应序列构建系统发育树并与以16S rRNA基因构建的系统发育树进行比较。结果表明:基于16S rRNA基因序列不能区分L.plantarum及其近缘种,而看家基因pheS和pyr G部分基因序列能够很好的区分植物乳杆菌种及其近缘种,其中,pheS在植物乳杆菌种内分型时相较于pyr G基因效果更好,以及将pheS和pyr G部分基因序列串联使用后,试验菌株与参考菌株的分类关系更加明晰,因此,联合基因(pheS-pyr G)可作为16S rRNA基因的辅助工具用于L.plantarum及近缘种和植物乳杆菌种内分型的快速分类鉴定。Phylogenetic trees of L. plantarum strains were constructed to explore the usage of the 16 S rRNA gene, pheS and pyr G gene sequences for distinguishing Lactobacillus plantarum from its related species.. The phylogenetic trees of ten L. plantarum strains isolated from sourdough, yoghurt, and pickles were constructed using partial sequences of housekeeping genes, pheS and pyrG, as molecular markers. These trees were compared with other phylogenetic trees constructed using the 16 S rRNA gene sequence of L. plantarum combined with the corresponding gene sequences of closely related strains already published in Gen Bank. The results showed that L. plantarum and its related species could be distinguished by analyzing the 16 S rRNA gene sequence; however, pheS and pyr G sequences could be used for this classification. Additionally, it was found that sequential use of pheS and pyr G could clearly distinguish strains from reference strains. Thus, a combination of genes（pheS-pyrG） could be used as an auxiliary tool along with 16 S rRNA gene analysis for rapid classification and identification of L. plantarum and related species.

关 键 词：植物乳杆菌 系统发育分析 16S RRNA基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]