复合诱变Penicillium purpurogenum Li-3选育高产红色素及其生物活性研究  

Screening of high red pigment-producing Penicillium purpurogenum Li-3 by compound mutagenese and its biological activity

作　　者：牛玉静[1] 曹红[2] 黄晓林[3] 童军茂[1] 隋佳慧 杨雨鑫[2] 

机构地区：[1]石河子大学食品学院,新疆石河子832003 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [3]浙江师范大学物理化学研究所,浙江金华321004

出　　处：《中国酿造》2017年第3期30-34,共5页China Brewing

基　　金：国家自然科学基金资助项目(21266029);浙江省自然科学基金资助项目(LY17B060011)

摘　　要：以产紫青霉菌(Penicillium purpurogenum)Li-3为出发菌株,采用紫外-氯化锂复合诱变方法选育高产红色素菌株;并对红色素进行生物活性研究,选取1,1-二苯基-2-苦肼基(DPPH)体系评价抗氧化活性;采用滤纸片扩散法研究产紫青霉菌突变株红色素对金黄色葡萄球菌(Staphylococcus aureus)和大肠杆菌(Escherichia coli)的抑制作用。结果表明,最佳紫外照射时间为120 s,最佳氯化锂质量分数为0.28%,筛选得到一株高产菌株P.purpurogenum Li-3-9,接种于液体发酵培养基中,32℃、150 r/min培养168 h后,测定其红色素色价最高值达到3.28 U/mL,比出发菌株提高了83%。且该菌株连续传代5次,其遗传稳定性较好。P.purpurogenum Li-3-9代谢产生的红色素具有较好的DPPH自由基清除能力和抑制金黄色葡萄球菌的能力。Using Penicillium purpurogenum Li-3 as original strain, the strain with high red pigment-producing ability was screened by ultraviolet （UV）- LiC1 compound mutagenesis, and the biological activities of red pigment were researched and evaluated by 2,2-diphenyl-l-picrylhydrazyl （DPPH） system. The inhibitory effects of red pigment produced by mutant strain on Staphylococcus aureus and Escherichia coli were studied by filter paper diffusion method. The results showed that optimal UV radiation time was 120 s and the optimal concentration of LiC1 was 0.28%, the P. purpuro- genum Li-3-9 with high red pigment-producing was obtained, inoculated into liquid fermentation medium and cultured at 32 ~C, 150 r/min for 168 h. The color value of red pigment reached the maximum value 3.28 U/ml, which was 83% higher than that of the original strain. After subculture of 5 successive generations, the strain had good genetic stability. The red pigment produced by P. purpurogenum Li-3-9 had better DPPH free radical scavenging capacity and inhibiting ability on S. aureus.

关 键 词：产紫青霉菌 复合诱变 红色素 生物活性 

分 类 号：Q815[生物学—生物工程]