高产洛伐他汀红曲霉(Monascus sp.UV-D-9)的发酵条件优化  

Optimization of fermentation conditions on lovastatin yield by Monascus sp.UV-D-9

作　　者：游玟娟[1] 温拥军[1] 

机构地区：[1]湖南化工职业技术学院,湖南株洲412004

出　　处：《食品工业科技》2017年第7期141-144,150,共5页Science and Technology of Food Industry

基　　金：湖南省教育厅科学研究项目(15C0466

摘　　要：为了进一步提高洛伐他汀产量,对实验组前期获得的洛伐他汀高突变株Monascus sp.UV-D-9的发酵条件进行了响应曲面优化。获得最佳发酵条件为:发酵温度30.4℃,装液量134 m L,转速187 r·min-1,其他条件为发酵时间10 d,接种量8%。在此条件下,洛伐他汀的产量为(0.32±0.014)mg·m L-1(n=3),比原始条件的产量提高了10.1%。The fermentation conditions of Monascus sp. UV - D - 9, which was a high - yield mutant obtained by complex mutagenesis, were optimized by Response Surface Methodology （RSM）to further improve lovastatin yield.The optimum fermentation conditions were received as follows：the fermentation temperature （X1）was 30.4 ℃, the medium volume （X2）was 134 mL, the rotation speed（ X3 ）was 187 r-rain-1, fermentation time was 10 d and inoculation amount was 8%. The lovastatin yield of Monascus sp.UV-D-9 under the optimum conditions was （ 0.32 ± 0.014） mg·mL^-1 （ n ： 3 ） , which was increased by 10.1% in comparison with the original condition.

关 键 词：洛伐他汀 红曲霉UV—D-9 响应曲面法 发酵条件 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]