复合酶法提取菊芋菊糖的工艺优化  

Optimization of Complex Enzymatic Extraction Technology of Inulin from Jerusalem artichoke

作　　者：江玉婷[1] 陈靖超[1] 李程程[1] 刘丽萍[1] 汪自慧 杭华[1] 

机构地区：[1]安徽师范大学,安徽芜湖241002

出　　处：《安徽农业科学》2017年第8期98-102,共5页Journal of Anhui Agricultural Sciences

基　　金：安徽师范大学2015年在校大学生创新创业训练计划项目(201510370025);2016年安徽省教育厅自然基金重点项目(611607);安徽师范大学"12博士科研启动项目"(161-070110)

摘　　要：[目的]优化复合酶提取菊芋菊糖的工艺。[方法]探讨料液比、pH、温度、复合酶等对菊芋菊糖提取率的影响,在此基础上,采用响应面分析方法,对其操作参数进行优化,获得最佳的工艺条件。[结果]菊芋菊糖提取的最佳工艺条件:料液比1∶20(g∶mL),pH 6.0,酶解温度54℃,复合酶(m_(木瓜蛋白酶)∶m_(果胶酶)=1∶8)131μg/g样品,提取时间40 min;在此条件下,菊芋菊糖提取率为73.30%。[结论]该研究可为菊芋菊糖的工业化生产奠定研究基础。[Objective]To optimize the complex enzymes extraction of inulin from Jerusalem artichoke.[Method]The effects of solid-liquid ratio,pH,temperature,enzyme contents on the yield of inulin were explored.On the basis of this,the response surface methodology was adopted to optimize operation parameters and obtain the best extraction conditions.[Result]The results showed that the optimum conditions were the radio of material-to-liquid of 1∶ 20（g∶mL）,pH 6.0,temperature of 54 ℃,compound enzymes（m_（papain）∶m_（pectinase）=1∶8） with enzyme sample of 131μg/g and extraction time of 40 mins.Under the optimum conditions,the inulin yield could obtain about 73.30%.[Conclusion]The study will provide the thesis basis for further industrial production.

关 键 词：菊芋 菊糖 复合酶法 响应面优化 提取 

分 类 号：S632.9[农业科学—蔬菜学] R284.2[农业科学—园艺学]