超声波辅助鱼皮胶原蛋白提取及其性质研究  

The ultrasonic-assisted solvent extraction and characterization of fishskin collagen

作　　者：王金梅[1] 包建强[1] 

机构地区：[1]上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《上海农业学报》2017年第2期114-119,共6页Acta Agriculturae Shanghai

基　　金：基金项目:水产动物遗传育种中心上海市协同创新中心(ZF1206);上海市科委工程中心建设(11DZ2280300)

摘　　要：利用超声波辅助提取草鱼皮胶原蛋白,并通过SDS-PAGE、圆二色谱法对超声作用得到的草鱼皮胶原蛋白结构和变性温度等进行了研究。通过响应面试验得到最优工艺条件:超声功率310 W,超声时间10 min,酶解时间29 h,提取率达到68.67%。SDS-PAGE和CD显示:胶原蛋白为典型Ⅰ型,且纯度较高,变性温度为36℃,胶原蛋白的三螺旋结构完整,超声波对其结构无明显影响,且具有生物活性。The ultrasonic-assisted solvent extraction of grass carp skin＇ s collagen was carried out, and the gained collagen＇ s structure and denaturing temperature were studied by SDS-PAGE and circular dichroism spectrocopy. The experiments by response surface methodology showed that the optimal processing conditions were 310-W ultrasonic power,10-min ultrasonication and 29-h enzymolysis ,in which the extraction rate reached 68.67%. The research by SDS-PAGE and circular dichroism speetroeopy indicated that the collagen was of Type I and had a comparatively high purity, its denaturing temperature was 36℃, and its triple-helix structure was intact and not influenced by ultrasonic wave, and also had a biological activity.

关 键 词：鱼皮 胶原蛋白 提取 超声波 凝胶电泳 圆二色谱 响应面分析 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]