采用2种降糖模型考察核桃蛋白及多肽的降糖作用  

Comparison of blood glucose model of Saccharomyces cerevisiae and animal

作　　者：李丽[1,2] 黄雪梦 杨璐嘉[1] 阮金兰[1,2] 刘阳[1,2] 

机构地区：[1]武昌理工学院生命科学学院生物多肽糖尿病药物湖北省协同创新中心湖北省生物多肽糖尿病药物工程技术研究中心,武汉430223 [2]武汉工程大学化工与制药学院,武汉430073

出　　处：《食品科技》2017年第4期218-221,共4页Food Science and Technology

基　　金：湖北省自然科学基金项目(2013CFB482);湖北省大学生创新训练项目(201312310012);武昌理工学院校级项目(2015X04)

摘　　要：目的:考察核桃蛋白及多肽的降糖活性,并比较酿酒酵母模型和T2DM动物模型优缺点。方法:采用酿酒酵母模型和T2DM动物模型,评价核桃蛋白及多肽降血糖活性。结果:在2种模型中,核桃蛋白与多肽均表现出一定降糖活性,并且核桃多肽的活性优于蛋白,实验结果在很大程度上表现出一致性。结论:酿酒酵母糖代谢模型实验周期短,经济、易于操作,可用于降糖物质筛选的前期选择。T2DM模型实验结果准确,在更大程度上接近类人型T2DM,可用于降糖物质的确定和深入研究。Objective： To investigate the hypoglycemic activity of walnut protein and peptides, and to compare the advantages and disadvantages of Saccharomyces cerevisiae and T2DM animal model. Methods： Using the model of Saccharomyces cerevisiae glucose metabolism and T2DM animal model, the activity of walnut protein and polypeptide was evaluated. Results： In the two models, walnut protein and peptide showed a certain hypoglycemic activity, and the activity of walnut polypeptide was better than that of protein, and the results of the experiment showed a great degree of consistency. Conclusion： Glucose metabolism of Saccharomyces cerevisiae model could be easy to operate, with short experimental cycle and lower cost, for blood glucose screening of early selection, T2DM model experimental results were accurate and in a greater degree of close type T2DM for hypoglycemic substances identified and in-depth study.

关 键 词：核桃蛋白 核桃多肽 降糖 T2DM模型 

分 类 号：TS201.21[轻工技术与工程—食品科学]