响应面法优化双孢蘑菇的酶解工艺  

Optimization of enzymatic hydrolysis of Agaricus bisporus by response surface method

作　　者：陈紫红[1] 吴燕[1] 花朋朋 赵立娜 方榕辉 林梅西 刘斌[1,2] 

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]国家菌草工程技术研究中心,福州350002 [3]福建省闽中有机食品有限公司,莆田351100

出　　处：《食品科技》2017年第4期222-228,共7页Food Science and Technology

基　　金：福建省科技重大专项(2014NZ2002-1);国家科技支撑计划子课题(2014BAD15B01);福建农林大学校杰青项目(xjq201608)

摘　　要：为优化双孢蘑菇酶解的最佳工艺条件,以水解度为指标,研究了酶的种类、酶解时间、料液比、pH、酶添加量和酶解温度对双孢蘑菇的水解度的影响。在单因素试验的基础上,通过响应面法对双孢蘑菇酶解工艺进行优化。结果表明:采用复合蛋白酶,最佳酶解条件为酶添加量1%(w:w)、料液比1:20(w:v)、温度50℃、时间8 h、pH8.0,在此条件下,双孢蘑菇酶解液的水解度达到(31.90±0.13)%,游离氨基酸总量约是酶解前的1.3倍,呈味氨基酸含量是酶解前的1.5倍。研究结果为双孢蘑菇的高值化利用及附加产品的开发提供了新的思路。To optimize technological conditions for enzymatic hydrolysis of Agaricus bisporus, the effect of enzymic species, hydrolysis time, material/liquid ratio, pH, enzyme dosage and temperature on the degree of hydrolysis （DH） of Agaricus bisporus were studied. Based on the single experiment, response surface method was employed to optimize conditions for the enzymatic hydrolysis of Agaricus bisporus. The optimal hydrolysis conditions were found as follows： enzyme dosage 1% （w：w）, material/liquid ratio 1：20 （w：w）, temperature 50 ℃, time 8 h, pH 8.0. Under this conditions, the DH of enzymatic hydrolysate of Agaricus bisporus was （31.90±0.13）%, and the total content of free amino acids of enzymatic hydrolyzate was increased to 1.3 times after hydrolysis. The results provided a reference for deep-processing and utilization of Agaricus bisporus.

关 键 词：双孢蘑菇 酶解 响应面 水解度 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]