发酵法生产DHA过程中油脂及DHA质量分数的快速定量测定  

Fast Quantitative Analysis of Lipids and DHA Contents During Fermentation Process for DHA Production

作　　者：赵祥颖[1,2] 赵晨[1,2] 张家祥[1,2] 杨丽萍[1,2] 刘建军[1,2] 田延军[1,2] 范宜晓[1,2] ZHAO Xiaiigyin ZHAO Chen ZHANG Jiaxian YANG Lipi LIU Jianjun TIAN Yanjun FAN Yixiao(Shandong Provincial Key Laboratory of Food and Fermentation Engineering,Jinan 250013,China Shandong Food Ferment Industry Research ＆ Design Institute, Jinan 250013 ,China)

机构地区：[1]山东省食品发酵工程重点实验室,山东济南250013 [2]山东省食品发酵工业研究设计院,山东济南250013

出　　处：《食品与生物技术学报》2017年第4期405-409,共5页Journal of Food Science and Biotechnology

基　　金：山东省科技发展计划项目(2014GSF121038)

摘　　要：针对发酵法生产DHA生产过程实时监控的需求,建立了一种简单、快速、准确的检测菌体细胞中油脂及DHA含量的方法。主要考察了菌体干燥,菌体细胞破壁方法、油脂抽提溶剂、油脂甲酯化方法以及内标选择等对菌体内油脂及DHA含量测定的影响等,确定了以湿菌体为分析样本,采用酸热法细胞破壁、正己烷抽提油脂、十九烷酸甲酯为内标、碱催化甲酯化测定发酵液中油脂及DHA含量方法,整个分析过程可在1~2 h内完成,特别适合DHA发酵过程的中间分析化验。A simple,fast and accurate method was established to determine the composition and contents of lipids and docosa-hexaenoic acid （DHA） during fermentation process for DHA production. The detection for lipids and DHA contents in Schizochytrium was optimized by drying methods, cell disruption methods, the ways of lipid extraction, the methods of methyl esterification and the selection of internal standards. The optimal analysis used an acid-heating method for cell disruption, n-hexane for lipid extraction,nonadecanoic acid methylester as the internal standard and methyl esterification catalyzed by alkali catalyst. It was particularly suitable for the intermediate analysis during fermentationprocess for DNA production whichcouldbe accomplished within 1 -2hours.

关 键 词：DHA 油脂分析 发酵控制 海洋真菌 

分 类 号：TQ645.6[化学工程—精细化工] TQ920.6[轻工技术与工程—发酵工程]