3种食用菌分级多糖消脂护肝功能研究  

Reduce Fat and Hepato-protective Studies on Fractional Polysaccharides of Pleurotus citrinipileatus,Auricularia polytricha and Grifola frondosa

作　　者：赵爽[1,2] 荣成博 刘宇[1,2] 陈杰[1] 徐甜甜[1] ZHAO Shuang RONG Cheng-bo LIU Yu CHEN Jie XU Tian-tian(Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China Key Laboratory of Urban Agriculture （North）, Ministry of Agriculture, Beijing 100097, China)

机构地区：[1]北京市农林科学院植物保护环境保护研究所北京市食用菌工程技术研究中心,北京100097 [2]农业部都市农业(北方)重点实验室,北京100097

出　　处：《食品研究与开发》2017年第8期158-162,共5页Food Research and Development

基　　金：北京市优秀人才培养资助项目(2015000020060G138);北京市农林科学院青年基金项目(QNJJ201524);北京市农林科学院科技创新能力建设专项(KJCX20170205)

摘　　要：通过热水提取、乙醇沉淀的方式获得榆黄菇、毛木耳和灰树花多糖,利用分子量截留的超滤方法获得15个分级多糖组分。采用酒精和脂肪酸(FFA)共同诱导Hep G2细胞建立脂肪肝模型,以细胞存活率和胞内甘油三酯含量为检测指标评价分级多糖消脂护肝作用。研究结果发现PS V、AP V和GF V 3个分子量高于100 k Da的组分是各自品种分级多糖中的优势组分,比例分别为71.06%、34%和68.89%;3种分级多糖可以提高肝损伤细胞的存活率分别为27.61%、37.68%和29.25%,同时减少甘油三酯在细胞内的堆积,具有明显的消脂护肝作用。This study obtained crude polysaccharides of Pleurotus citrinipileatus,Auricularia polytricha and Grifola frondosabythe hot-water-extraction and ethanol-precipitation method. The crude polysaccharides were divided into 15 fractional components using ultrafiltration method of molecular weight. Fatty liver injury model was induced by alcohol and fatty acids with Hep G2 cells. The cell survival rate and intracellular triglyceride levels were detected to evaluate the hepato-protective effect of fractional polysaccharides. The results showed that the components PS V,AP V and GF V,molecular weight above 100 k Da,were the highest content fractions each strains with the ratios of 71.06 %,34 % and 68.89 %,respectively. PS V,AP V and GF V could increase the survival rate of injury hepatic cells,which were 27.61 %,37.68 % and 29.25 %,respectively. Meanwhile these fractional polysaccharides also reduced intracellular triglycerides accumulation,which indicated that they were potential agents in hepatic protection application.

关 键 词：榆黄菇 毛木耳 灰树花 多糖 消脂 护肝 

分 类 号：R151[医药卫生—营养与食品卫生学]