豆粕多糖的酶法提取工艺研究  

Study on the Enzymatic Extraction Process of Polysaccharides from Bean Pulp

作　　者：范文奇[1] 要志宏[1] 申丽媛[1] 聂相珍 李波[1] 

机构地区：[1]扬州大学旅游烹饪学院,江苏扬州225127

出　　处：《中国调味品》2017年第5期65-68,83,共5页China Condiment

摘　　要：试验利用纤维素酶提取豆粕多糖,并利用苯酚-硫酸法测定样品中多糖的含量,选取料液比、酶解温度、酶解时间、pH值、纤维素酶添加量为试验条件,通过试验确定单因素的最佳试验条件,同时在单因素试验的基础上进行正交试验,确定最佳试验组合。两次试验结果显示:酶法提取豆粕多糖的最适试验条件为酶解时间90min,酶解温度60℃,pH 5.0,纤维素酶添加量1.0%,料液比1∶20,多糖得率达到14.92%。Use cellulase to extract bean pulp polysaccharides, and then adopt phenol-sulfuric acid method to determine the content of bean pulp polysaccharides. Choose the ratio of solid to liquid, enzymatic temperature, enzymatic time, pH, cellulase amount as experimental conditions, and on the basis of single factor experiment, use orthogonal experiment to determine the optimum combination of factors. The experimental results show that the optimum experimental conditions for bean pulp polysaccharides are enzymatic reaction time of 90 min, enzymatic temperature of 60℃, pH of 5. 0, cellulase amount of 1.0M, the ratio of solid to liquid of 1 ： 20, the polysaccharides extraction rate reaches 14.92%.

关 键 词：提取工艺 豆粕多糖 纤维素酶 苯酚一硫酸法 

分 类 号：TS201.1[轻工技术与工程—食品科学]