温度对高温大曲液态培菌过程菌群结构的影响  

Effect of temperature on microbial composition during high temperature Daqu liquid cultivation

作　　者：邱增钰 王亚平[1] 李博艺 谢彪 刘国峰 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457 [2]金开生物工程有限公司,四川成都611130 [3]尤特尔生物科技有限公司,山东邹城273500

出　　处：《中国酿造》2017年第5期30-35,共6页China Brewing

基　　金：国家高技术研究发展计划‘863计划’项目(2012AA022108);中国白酒3C计划项目(1400040024)

摘　　要：选用高温大曲在30℃、37℃、40℃、43℃和53℃不同温度条件下液体培养,对培养过程中微生物菌群结构和代谢产物的变化进行了分析。结果表明,较低的培养温度有利于酵母菌和乳酸菌的生长,而较高的培养温度有利于耐高温细菌和霉菌的生长。其中,酵母菌在37℃培养60 h数量最多,霉菌在53℃培养48 h数量最多,乳酸菌在37℃培养24 h数量最多,总细菌在40℃培养24 h数量最多。从代谢产物的分析结果看,酒精度和总酸含量在37℃时含量最高,乙酸乙酯、总酯含量在30℃时最高,α-氨基酸态氮含量在53℃时最高。可根据增强发酵活力、提高酒度和酯含量等需求,延长不同培养温度范围的保持时间。Effect of temperature on microbial composition and metabolism during liquid cultivation of high temperature Daqu at 30 ℃, 37 ℃, 40 ℃, 43 ℃, 53 ℃ was investigated. Results showed that low temperature cultivation was beneficial to the growth of yeast and lactic acid bacteria, and high temperature was beneficial to the growth of the high temperature tolerant bacteria and mycetes. The yeast count was the largest at 37 ℃ for 60 h cultivation, the mycetes count was the largest at 53 ℃ for 48 h cultivation, the lactic acid bacteria count was the largest at 37 ℃ for 24 h cultivation, and the total bacteria count was the largest at 40 ℃ for 24 h cultivation, From the analysis results of metabolites, alcohol content and total acid content were the highest at 37 ℃, ethyl acetate and total ester contents were the highest at 30 ℃, and α-amino nitrogen content was the highest at 53 ℃. So the time can be prolonged to maintain different temperature range, in order to enhance different requirements, such as to enhance fermentation activity and to improve alcohol content and ester content, etc.

关 键 词：高温大曲 菌群结构 代谢产物 液态培养 

分 类 号：TS261.1[轻工技术与工程—发酵工程]