柿叶茶、杜仲雄花茶、绿茶和红茶体外抗氧化活性比较研究  

Comparative Studies on the Antioxidant Activity of Persimmon Leaf Tea,Eucommia ulmoides Male Flower Tea,Green Tea and Black Tea in vitro

作　　者：韩卫娟[1,2] 张嘉嘉[1,2] 杜改改[1,2] 孙鹏[1,2] 刁松锋[1,2] 傅建敏[1,2] 

机构地区：[1]国家林业局泡桐研究开发中心,河南郑州450003 [2]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《西北林学院学报》2017年第3期144-148,155,共6页Journal of Northwest Forestry University

基　　金：国家"十二五"农村领域科技计划(2013BAD14B0502)

摘　　要：为了解柿叶茶、杜仲雄花茶、绿茶和红茶抗氧化活性的差异性,采用AlCl_3-(HAc-NaAc)、Folin-Ciocalteu和HPLC法对黄酮、总酚和维生素C含量进行分析,通过DPPH·,ABTS+·,还原能力(Fe^(3+)和Cu^(2+)),金属络合能力(Fe^(2+)和Cu^(2+))6种方法对其体外抗氧化作用进行了比较研究。结果表明,柿叶茶富含维生素C,杜仲雄花茶黄酮含量最高,绿茶和红茶中活性成分主要是茶多酚;4种茶具有显著的体外抗氧化能力,除络合能力外,绿茶、红茶均强于柿叶茶和杜仲雄花茶,抗氧化能力与总酚含量呈极显著的正相关关系,与黄酮含量无显著的相关性。因此,4种茶富含黄酮、多酚、维生素C等天然抗氧化成分,且具有显著的抗氧化活性。In order to systematically evaluate the differences in antioxidant activity among four types of tea：persimmon leaf tea,Eucommia ulmoides male flower tea,green tea and black tea in vitro,the contents of total flavonoids,polyphenols and vitamin C in the samples were measured AlCl3-（HAc-NaAc）,Folin-Ciocalteu,and HPLC methods.The antioxidant activities were investigated by measuring the capabilities of scavenging DPPH·,ABTS＋· free radicals,reducing power （Fe3＋＆Cu2＋）,metal-chelating assays （Fe2＋＆Cu2＋.It was found that there were abundant vitamin C in persimmon leaf tea,the total flavonoid content in E.ulmoides male flower tea was the highest,and polyphenols was the principal group of active components in green and black tea.Four kinds of tea all showed excellent antioxidant activity in vitro,and green tea and black tea were better than persimmon leaf tea except metal-chelating assays.The significant correlation was observed between polyphenol content and antioxidant activity,but the correlation between flavonoid content and antioxidant activity was not significant.

关 键 词：柿叶茶 杜仲雄花茶 绿茶 红茶 抗氧化活性 

分 类 号：S713[农业科学—林学]